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为明确河南省小麦根腐线虫病的发生危害情况及其病原线虫种类,对14个县(市)45个采样点小麦根腐线虫进行了分离调查,分别通过人工接种测定其致病性和杀线剂处理研究其危害性,并采用形态学和分子生物学方法鉴定其病原。结果显示:小麦根腐线虫在河南省的分布具有一定的区域性,中北部的10个县(市)34个沙质土壤采样点均分离出小麦根腐线虫,而在南部土壤粘重地区的小麦根内和土壤中均没有分离出根腐线虫;15%涕灭威颗粒剂处理土壤后小麦的穗粒数、千粒重和单位面积产量较对照明显上升,产量增加13.6%;该线虫能够侵染小麦,并明显抑制小麦生长;河南省小麦根腐线虫以敏捷短体线虫Pratylenchus agilis为主。
In order to clarify the occurrence and the pathogenic nematodes of wheat root rot nematode disease in Henan Province, 45 samples of wheat root rot nematodes in 14 counties (cities) were isolated and investigated, respectively, by artificial inoculation to determine the pathogenicity and killing The agent is studied for its harmfulness, and the pathogen is identified by morphological and molecular biological methods. The results showed that the distribution of wheat root rot nematode in Henan Province was of a certain regionality. The root rot nematodes of wheat were isolated from 34 sandy soil sampling sites in 10 counties (cities) of the central and northern part of China. However, The root rot nematodes were not isolated from wheat roots and soil. The grains per spike, 1000-grain weight and yield per unit area of wheat after 15% aldicarb granules treatment significantly increased compared with the control and the yield increased by 13.6%. The nematodes were able to infect Wheat, and obviously inhibited the growth of wheat. The root rot nematode of wheat in Henan province was mainly Pratylenchus agilis.