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目的初步探讨龙葵碱对睾丸支持细胞的毒性。方法原代培养睾丸支持细胞,MTT法检测不同浓度龙葵碱对于支持细胞的毒性作用;激光共聚焦显微镜观察细胞内线粒体膜电位的变化以及[Ca2+]i的改变。结果龙葵碱作用于睾丸支持细胞72h的IC50为22.29μmol/L。随着剂量的增大,细胞内线粒体膜电位降低,[Ca2+]i升高。结论龙葵碱通过损伤小鼠睾丸支持细胞线粒体,升高细胞[Ca2+]i,从而对睾丸支持细胞产生毒性作用。
Objective To study the toxicity of Solanum nigrum on testicular Sertoli cells. Methods Primary cultured testicular Sertoli cells were cultured. The toxicity of solanine against different levels of Sertoli cells was detected by MTT assay. Confocal laser scanning microscopy was used to observe the change of intracellular mitochondrial membrane potential and the change of [Ca2+]i. RESULTS: The IC50 of nigrum base on testicular Sertoli cells was 22.29 μmol/L for 72 h. As the dose increased, the intracellular mitochondrial membrane potential decreased and [Ca2+]i increased. Conclusion Solanine can damage the testis support cells by injuring the mitochondria of the testis and increasing the [Ca2+]i of the cells.