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<正>Sea urchin Toxopneustes pileolus belongs to the family Toxopneustidae,is one of the toxic animal which mainly distribute in the subtropical regions of Japan.Since the pedicellariae of this sea urchin contained toxins and often causespenetrated injury to animal,it’s important to understand the toxin effect to human.In this experiment,30 sea urchins were used here for toxin extraction.Sea urchins were collected during March,2012.The pedicellariae of each sea urchin