目的探讨腹腔镜腹膜阴道成形术在临床应用方面的相关问题。方法以罗湖医院首创的腹腔镜腹膜阴道成形术(罗湖一式)为前提,不断改进并完善手术方法,将其逐渐衍变为罗湖二式。结果对316例先天性无阴道(Mayer-Rokitanskyl-Küster-Hauser,MRKH)综合征患者实施罗湖二式手术,效果良好。结论罗湖二式腹腔镜腹膜阴道成形术是目前理想的一种治疗MRKH的手术方法,值得临床广泛推广。 Objective To investigate the clinical application of laparoscopic peritoneal vaginoplasty. Methods Based on the first laparoscopic Laparoscopic vaginoplasty (Lo Wu-type) in Luohu Hospital, we continuously improved and perfected the operation method and gradually changed it to Luohu II. Results A total of 316 patients with Mayer-Rokitanskyl-Küster-Hauser syndrome (MRKH) syndrome were treated with Luohu II-type surgery, with good results. Conclusion Luo laparoscopic laparoscopic peritoneal vaginoplasty is the ideal method for the treatment of MRKH surgery, it is worth widely clinical promotion.