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目的本实验利用APP/PS1双转基因模型小鼠,观察隐丹参酮对小鼠行为学习及抗氧化能力的影响。方法选用APP/PS1双转基因模型小鼠,将其随机为5组,另取同窝阴性小鼠作为正常对照组,每组10只,3个月龄后开始给药。给药4个月后,通过行为学实验测试小鼠学习记忆能力的变化;采用分光光度法测定血清和脑组织中谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)活性和丙二醛(MDA)的含量。结果 Morris水迷宫实验显示,APP/PS1双转基因模型小鼠逃避潜伏期较正常组明显延长,原象限停留时间明显缩短。隐丹参酮组小鼠逃避潜伏期较模型组明显缩短,原象限停留时间明显延长。APP/PS1双转基因模型小鼠血清和脑组织GSH-Px、SOD活力下降,MDA含量明显增多;隐丹参酮治疗组学习和记忆能力升高,血清和脑组织GSH-Px、SOD活力升高,MDA含量减少,与阿尔茨海默病模型组比较有显著性差异。结论 APP/PS1双转基因模型小鼠7个月后发展有明显学习记忆功能障碍伴有抗氧化能力的下降,隐丹参酮对阿尔茨海默病模型小鼠学习记忆障碍具有明显改善作用并且可剂量依赖性的显著改善阿尔茨海默病模型小鼠的抗氧化能力。
Objective In this experiment, APP / PS1 double transgenic mice were used to observe the effect of cryptotanshinone on behavioral learning and antioxidant capacity in mice. METHODS: APP / PS1 double transgenic mice were randomly divided into five groups. Another littermate negative control group was used as normal control group, with 10 mice in each group and 3 months old. After 4 months of administration, the learning and memory abilities of mice were tested by behavioral experiments. The levels of glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) in serum and brain tissue were measured by spectrophotometry ) Activity and malondialdehyde (MDA) content. Results Morris water maze test showed that APP / PS1 double transgenic model mice evidently prolonged the escape latency compared with the normal group, and the original quadrant residence time was significantly shortened. Cryptotanshinone mice escape latency was significantly shorter than the model group, the original quadrant of the residence time was significantly prolonged. GSH-Px, SOD activity in serum and brain tissue of APP / PS1 double transgenic mice decreased and the content of MDA increased significantly. The learning and memory abilities of GSH-Px and SOD in serum and brain tissue increased significantly in cryptotanshinone treatment group Content reduced, and Alzheimer’s disease model group compared with significant differences. Conclusions The APP / PS1 double transgenic mouse model developed obvious learning and memory deficits accompanied by decreased antioxidant capacity after 7 months. Cryptotanshinone significantly improved learning and memory deficits in mice with Alzheimer’s disease and was dose-dependent Sexually Significantly Improved Antioxidant Capacity in Alzheimer’s Disease Model Mice.