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目的研究吡咯喹啉醌(pyrroloquinoline quinone,PQQ)对受到氧化损伤的神经细胞的修复作用,探讨PQQ在因衰老产生机体氧化损伤的大鼠体内、脑内的抗氧化作用,以及该抗氧化作用对衰老大鼠学习记忆能力产生的影响。方法使用H_2O_2诱导PC12神经细胞(大鼠肾上腺髓质嗜铬瘤分化细胞株)氧化损伤,随后用噻唑兰法检测PQQ对PC12的修复作用。用PQQ(0、10、20、40 mg/kg)灌胃18月龄雄性SD大鼠,4周后用Morris水迷宫试验测定大鼠的学习记忆能力,6周后测定血清和脑组织的氧化损伤水平和抗氧化能力。结果 200 nmol/L的PQQ使PC12细胞的存活率从59.1%增加到90.5%;与衰老模型组比较,PQQ中和高剂量组(20、40 mg/kg)大鼠在Morris水迷宫试验中的5 d潜伏期缩短、5 d游泳总路程减少,PQQ各剂量组(10、20、40 mg/kg)7 d穿越次数增加、7 d第一次平台穿越时间减少。同时PQQ各剂量组大鼠血清和脑组织中丙二醛、脑组织中脂褐素水平降低,中和高剂量组(20、40 mg/kg)血清和脑组织中超氧化物歧化酶水平、谷胱甘肽过氧化物酶活力以及脑组织中抗氧化能力增强。结论 PQQ可修复神经细胞的氧化损伤,证实了PQQ能够在体内与大脑中同样发挥抗氧化作用,增加衰老大鼠学习记忆能力。
Objective To investigate the effect of pyrroloquinoline quinone (PQQ) on the repair of oxidative damaged neurons and to explore the antioxidative effect of PQQ in vivo and in vivo on the oxidative damage of oxidative stress induced by aging in rats. Effects of Learning and Memory in Aging Rats. Methods The oxidative damage of PC12 cells (rat adrenal medullary pheochromocytoma cell line) was induced by H_2O_2, and then the repair effect of PQQ on PC12 was detected by thiazolyl method. Male SD rats 18 months old were intragastrically administrated with PQQ (0, 10, 20, 40 mg / kg), the learning and memory abilities of rats were measured by Morris water maze test after 4 weeks and the oxidation of serum and brain tissue Injury and antioxidant capacity. Results PQQ at 200 nmol / L increased the survival rate of PC12 cells from 59.1% to 90.5%. Compared with aging model group, the rats in PQQ medium and high dose group (20,40 mg / kg) The latency of 5 days was shortened, and the total distance of swimming on 5 days was decreased. The number of traversing of PQQ at each dose group (10, 20, 40 mg / kg) increased on the 7th day and decreased on the 7th day after the first platform crossing. At the same time, the levels of lipofuscin in serum and brain tissue of PQQ rats were decreased, while the level of superoxide dismutase in serum and brain tissue of moderate and high dose groups (20 and 40 mg / kg) Glutathione peroxidase activity and antioxidant capacity in brain tissue increased. Conclusion PQQ can repair the oxidative damage of nerve cells and confirm that PQQ can also exert anti-oxidant effect in vivo and in the brain and increase the learning and memory ability of aged rats.