吡咯喹啉醌对衰老大鼠学习记忆的影响及机制研究

来源 :中国食品卫生杂志 | 被引量 : 0次 | 上传用户:wsf3344
下载到本地 , 更方便阅读
声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架
论文部分内容阅读
目的研究吡咯喹啉醌(pyrroloquinoline quinone,PQQ)对受到氧化损伤的神经细胞的修复作用,探讨PQQ在因衰老产生机体氧化损伤的大鼠体内、脑内的抗氧化作用,以及该抗氧化作用对衰老大鼠学习记忆能力产生的影响。方法使用H_2O_2诱导PC12神经细胞(大鼠肾上腺髓质嗜铬瘤分化细胞株)氧化损伤,随后用噻唑兰法检测PQQ对PC12的修复作用。用PQQ(0、10、20、40 mg/kg)灌胃18月龄雄性SD大鼠,4周后用Morris水迷宫试验测定大鼠的学习记忆能力,6周后测定血清和脑组织的氧化损伤水平和抗氧化能力。结果 200 nmol/L的PQQ使PC12细胞的存活率从59.1%增加到90.5%;与衰老模型组比较,PQQ中和高剂量组(20、40 mg/kg)大鼠在Morris水迷宫试验中的5 d潜伏期缩短、5 d游泳总路程减少,PQQ各剂量组(10、20、40 mg/kg)7 d穿越次数增加、7 d第一次平台穿越时间减少。同时PQQ各剂量组大鼠血清和脑组织中丙二醛、脑组织中脂褐素水平降低,中和高剂量组(20、40 mg/kg)血清和脑组织中超氧化物歧化酶水平、谷胱甘肽过氧化物酶活力以及脑组织中抗氧化能力增强。结论 PQQ可修复神经细胞的氧化损伤,证实了PQQ能够在体内与大脑中同样发挥抗氧化作用,增加衰老大鼠学习记忆能力。 Objective To investigate the effect of pyrroloquinoline quinone (PQQ) on the repair of oxidative damaged neurons and to explore the antioxidative effect of PQQ in vivo and in vivo on the oxidative damage of oxidative stress induced by aging in rats. Effects of Learning and Memory in Aging Rats. Methods The oxidative damage of PC12 cells (rat adrenal medullary pheochromocytoma cell line) was induced by H_2O_2, and then the repair effect of PQQ on PC12 was detected by thiazolyl method. Male SD rats 18 months old were intragastrically administrated with PQQ (0, 10, 20, 40 mg / kg), the learning and memory abilities of rats were measured by Morris water maze test after 4 weeks and the oxidation of serum and brain tissue Injury and antioxidant capacity. Results PQQ at 200 nmol / L increased the survival rate of PC12 cells from 59.1% to 90.5%. Compared with aging model group, the rats in PQQ medium and high dose group (20,40 mg / kg) The latency of 5 days was shortened, and the total distance of swimming on 5 days was decreased. The number of traversing of PQQ at each dose group (10, 20, 40 mg / kg) increased on the 7th day and decreased on the 7th day after the first platform crossing. At the same time, the levels of lipofuscin in serum and brain tissue of PQQ rats were decreased, while the level of superoxide dismutase in serum and brain tissue of moderate and high dose groups (20 and 40 mg / kg) Glutathione peroxidase activity and antioxidant capacity in brain tissue increased. Conclusion PQQ can repair the oxidative damage of nerve cells and confirm that PQQ can also exert anti-oxidant effect in vivo and in the brain and increase the learning and memory ability of aged rats.
其他文献
<正>提起如何打造模具品牌效益这个话题,把这一两年来我分厂的销售订单进行分类汇总后就能看出:模具产品转型的决策已经取得了成效,我们在高端电动机模具行业中站住了脚,有了
随着我国课程改革的不断深入,小学阶段的教学不仅单纯地注重学生的各科学习成绩,更注重对学生综合素质的培养.因此,在我国不仅对小学生开展了义务教育,还要求在小学阶段对学
党建是党的建设的简称。党的建设是党主要的工作概念,又是对党的日常事务处理的概念。党建是指执政党为了保持自己原有的性质而在建党之后进行一系列的自我完善活动,不仅包括
目的:讨论在临床上对重度癌因性疲乏的肺癌化疗患者的临床护理对策,分析其临床运用价值。方法:按照肺癌化疗顺序将66例伴有重度癌因性疲乏患者按1∶1比例分为两组,对照组进行常
高职共享型实训基地建设呈现出重硬件轻软件、重建设轻管理的发展趋势,并导致实训资源共享不足、常态建设机制欠缺、日常维护难度加大等问题。在"互联网+"背景下,基于云计算、大