Gliomas, which accounts for more than 50% as one of the primary tumors of the nervous system, is the most common primary central nervous system malignancies and also one of the most aggressive malignant tumors.There are about 90,000 new patients per year in China.With the in-depth understanding of the mechanism of glioma molecular biology pathogenesis, people gradually realized the occurrence and development of glioma is a multi-gene involvement, multi-link, multi-stage and multi-step complex process. The truth has now been found that inactivation of functional tumor suppressor genes such as p53,Rb, P16 etc.and over-expression of nuclear transcription factors such as FoxM1,MDM2,cdc2, VEGF,Survivin etc. can lead the tumor cells apoptosis suppressed and unlimited growth. So we need to find some of the key nuclear transcription factors, which can regulate multiple downstream tumor-associated genes or the expression of apoptosis-related genes.FoxMl is a proliferation-specific transcription factor. After FoxM1 protein activated, it can regulate many key factors with the G1/S phase, G2/M phase transition and mitotic cell cycle regulation. The research found that FoxM1 was continuously and highly expressed in many cancers including glioma. Over-expression FoxM1 will disorder downstream target genes, such as Survivin, Skp2, which can protect apoptosis, enhance cell proliferation, and induce the tumor cells out of control in cell growth. Studies have proved that FoxM1 makes high expression of angiogenic factors VEGF and promote glioma growth. In short, FoxM1 might be a potential target sites of the glioma’s gene therapy.In this paper, we used RNA interference to block the FoxM1 signaling pathway, and studied the effect and mechanism of the inhibition of glioma proliferation. Therefore, we constructed Ad hFoxM1 siRNA, and got a large number of high-titer adenovirus by using the technolog of CsCl density gradient centrifugation. Make H4 and A172 cell infected by the adenovirus and the result showed that the adenovirus could successfully eliminate the expression of FoxM1 in the mRNA level and at the protein level by using fluorescence quantitative PCR and western-blot demonstrating. Then we made the cell growth curve, found that the two cell proliferation had been inhibited. Further, we detected the mRNA level of cell cycle related genes Skp2 and CDC25B,apoptosis related genes Apaf-1 and inhibition of apoptosis related genes Survivin. We found that in A172 cell line which FoxM1 was knocked out, the mRNA level of Skp2, CDC25B and Survivin was significantly reduced in contrast to Apaf-1 significantly increased, suggesting that cell proliferation had been inhibited and cell apoptosis increased. In summary, the results showed that FoxM1 was an important factor to the formation and development of glioma. All the results still to be confirmed in vivo and clinical.