本文建立了简便、快速的分离测定辣椒干中辣椒素含量的高效液相色谱法。HPLC的条件:Burospher100C18柱(I.D.250mm×4.6mm,5μm);流动相为甲醇水(70∶30,V/V);流速为0.6mL/min;检测波长为280nm。在上述条件下,辣椒素在1.01～121.2mg/L的范围内线性关系良好,线性相关系数γ=0.9994;实验结果表明,该方法的相对标准偏差在1.38%(n=6)以内,平均回收率为99.88%。 In this paper, a simple and rapid HPLC method was developed for the determination of capsaicin in dried peppers. HPLC conditions: Burospher100C18 column (I.D. 250 mm × 4.6 mm, 5 μm); the mobile phase was methanol water (70:30, V / V); the flow rate was 0.6 mL / min; the detection wavelength was 280 nm. Under the above conditions, the linearity of capsaicin in the range of 1.01 ~ 121.2mg / L was good with linear correlation coefficient γ = 0.9994. The experimental results showed that the relative standard deviation of this method was within 1.38% (n = 6) The rate is 99.88%.