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为明确甜菜过氧化物酶cprx1基因在抗旱节水中的功能,利用甜菜品种HI0466(抗旱性较强)、农大甜研4号(抗旱性较弱)为材料,通过已克隆的甜菜cprx1基因序列设计引物,利用半定量RT-PCR方法,对甜菜幼苗根系、叶片中cprx1基因在正常供水及PEG6000模拟水分胁迫1d、3d、5d及复水1d、2d时的表达模式进行分析。结果显示,2个甜菜品种幼苗根、叶中cprx1基因在正常供水情况下均有一定量的表达;在水分胁迫1d均诱导上调表达;水分胁迫至第3天HI0466根、叶中表达量显著增强,而农大甜研4号根、叶中该基因表达受到抑制;胁迫至第5天HI0466根、叶中该基因仍有微量表达,而农大甜研4号根、叶内该基因表达接近停止;复水2d后表达量均恢复至胁迫前水平。
In order to clarify the function of cprx1 gene in drought-resistant and water-saving, the sugar beet cultivar HI0466 (strong drought resistance) and Nongda Tianyan No.4 (drought-resistant) were used as material to screen the cprx1 gene sequence of beet Primers were designed to analyze the expression pattern of cprx1 gene in sugarbeet seedling roots and leaves under normal water supply and PEG6000 simulated water stress 1 d, 3 d, 5 d and 1 d, 2 d after rewatering by semi-quantitative RT-PCR. The results showed that the cprx1 gene in seedling roots and leaves of two beet varieties all expressed in a certain amount of water under normal water supply conditions and up-regulated at 1 d of water stress. The expression level of cprx1 gene in leaves and roots of three sugar beet seedlings increased significantly on day 3, On the other hand, the gene expression was inhibited in root of Nongda Tianyan 4, and the gene was still slightly expressed in the leaves of HI0466 on the 5th day after stress. However, After 2 days of water, the expression levels recovered to the level before stress.