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为了改进扫描电镜样品制备技术,对比观察了甲醛、戊二醛、戊二醛—锇酸等3种不同固定方法对气管粘膜的固定效果,现简报如下。1 技术过程 按照常规扫描电镜样品的取材和清洗方法,将同一鸡气管的样品切割成适当大小,随机分成3组。第1组用10％甲醛固定;第2组用2.5％戊二醛固定;第3组用戊二醛—锇酸双重固定。固定后,脱水、临界点干燥及喷涂金钯等条件3组均同。用SE—570扫描电镜观察。
In order to improve the scanning electron microscopy sample preparation technology, comparative observation of formaldehyde, glutaraldehyde, glutaraldehyde - osmotic acid and other three fixed methods of tracheal mucosa fixation effect are as follows. A technical process According to conventional scanning electron microscopy sample preparation and cleaning methods, the same chicken trachea samples cut into appropriate size, were randomly divided into three groups. Group 1 was fixed with 10% formaldehyde; Group 2 was fixed with 2.5% glutaraldehyde; Group 3 was double-fixed with glutaraldehyde-osmic acid. After fixing, dehydration, critical point drying and spraying gold palladium and other conditions are the same 3 groups. Using SE-570 scanning electron microscope.