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目的：建立改良香荚兰素比色法测定茶多酚口含片中儿茶素含量。方法：取样品乙醇液４０μｌ，置于５ｍｌ容量瓶中，分别加水１．０ｍｌ，１％香荚兰素浓盐酸液加至刻度，摇匀，４０ｍｉｎ后，于５０５ｎｍ测定吸收度，在７．５８～５３．０５μｇ线性关系良好（ｒ＝０．９９９８）。结果：口含片儿茶素平均标示量９９．３５％，ＲＳＤ１．０３％。结论：该法简便，快速，辅料无干扰，可用于原料药和制剂的含量分析。
OBJECTIVE: To establish an improved method for the determination of catechins in tea polyphenols buccal tablets by colorimetry with vanillin. Methods: Take 40μl of ethanol sample, place it in a 5ml volumetric flask, add 1.0ml of water and add 1% vanillin concentrated hydrochloric acid solution to the mark. Shake well. After 40min, measure the absorbance at 505nm, at 7.58. The 53.05 μg linearity was good (r=0.9998). Results: The average amount of labeled catechin was 99.35% and RSD was 1.03%. Conclusion: This method is simple, rapid and without interference of excipients. It can be used for the analysis of content of drug substances and preparations.