论文部分内容阅读
目的:胰高血糖素样肽1(GLP-1)在心血管系统发挥重要作用,本实验着重研究二肽酶4抑制剂sitagliptin是否通过增加GLP-1、抑制内质网应激而改善肥胖小鼠的血管内皮功能。方法:高脂饮食喂饲C57BL/6小鼠4个月诱导肥胖,在第4个月给予sitagliptin灌胃。利用微血管测定技术观察离体主动脉环对乙酰胆碱的累积舒张反应;采用Western blot检测相关靶蛋白的
OBJECTIVE: Glucagon-like peptide 1 (GLP-1) plays an important role in the cardiovascular system. In this study, we focused on whether sitagliptin, a dipeptidase 4 inhibitor, ameliorates obese mice by increasing GLP-1 and inhibiting endoplasmic reticulum stress The vascular endothelial function. METHODS: C57BL / 6 mice fed a high-fat diet for 4 months induced obesity and were given sitagliptin orally at 4 months. The microvascular technique was used to observe the cumulative relaxation response of isolated aortic rings to acetylcholine. Western blot was used to detect the relative target proteins