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目的评估成年大鼠冻存卵巢组织自体异位移植后的长期生殖、内分泌功能。方法78只Wistar成年大鼠,随机分为假手术组(1组,15只)、新鲜组织移植组(2组,24只)、冻存组织移植组(3组,24只)、去势组(4组,15只)。将去势后成年大鼠的新鲜卵巢组织及冻存卵巢组织自体异位移植于肾被膜下,分别于移植后5、8、10个月处死各处理组1/3数量动物,取卵巢及子宫组织作组织学检查。通过阴道脱落细胞学检查、动情期雌、孕激素水平监测评估移植后卵巢组织的生殖内分泌功能。结果所有动物模型中,新鲜及冻存卵巢组织移植后都可观察到存活组织块。2、3组动情期雌、孕激素水平与假手术组无显著性差异(P>0.05),且均明显高于去势组(P<0.01)。冷冻复苏后组织与新鲜组织各级卵泡构成比无显著性差异(P>0.05),各组不同时间取得的卵巢组织各级卵泡构成比相比较均无显著性差异(P>0.05)。移植后5个月,2、3组原始卵泡明显减少,分别为假手术组的59.1%和54.5%。结论小块卵巢组织可耐受冻融过程;冻存卵巢组织自体肾被膜下移植后,卵巢组织形态无明显改变,原始卵泡总数虽然明显减少,但有成熟卵泡发育并排卵,并能长期维持生殖内分泌功能。
Objective To evaluate the long-term reproductive and endocrine function of adult rat cryopreserved ovarian tissue after autologous xenotransplantation. Methods Totally 78 Wistar adult rats were randomly divided into sham operation group (group 1, group 15), fresh tissue transplantation group (group 2, group 24), frozen tissue transplantation group (group 3, group 24) (4 groups, 15). The ovariectomized adult ovariectomized ovariectomized rats were autotransplanted into the subrenal capsule, and the animals were sacrificed 5, 8, and 10 months after transplantation. One third of animals in each treatment group were sacrificed and the ovaries and uterus Organization for histological examination. Vasogenic and endocrine functions of ovarian tissue after transplantation were evaluated by vaginal cytology, estrus estrogen and progesterone monitoring. Results In all animal models, surviving tissue mass was observed after fresh and frozen ovarian tissue was transplanted. The levels of estrogen and progesterone in estrogen and estrogen of groups 2 and 3 were not significantly different from those of sham operation group (P> 0.05), and were significantly higher than that of castrated group (P <0.01). There was no significant difference in constituent ratio of follicles between fresh tissues and tissues after freezing and resuscitation (P> 0.05). There was no significant difference in the constituent ratio of follicles at all stages between different groups (P> 0.05). At 5 months after transplantation, the primordial follicles in groups 2 and 3 were significantly reduced, which were 59.1% and 54.5% of the sham group respectively. Conclusion Small ovarian tissue can tolerate the process of freezing and thawing. After transplanted into the frozen subrenal tissue, there is no obvious change in ovarian tissue morphology. Although the total number of primordial follicles is significantly reduced, mature ovarian follicles develop and ovulate, Endocrine function.