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在直流10V电压下电解聚乙烯醇和硝酸银的混合液3h制备纳米银胶,将经过半胱氨酸修饰后的载玻片浸入银胶24h制得纳米银膜。用紫外可见分光光度计对银胶进行了观测,由于其吸收峰半高宽较窄可知银胶中纳米银颗粒粒径分布较为均匀。同时,使用扫描电镜对银膜进行了表征。通过对半胱氨酸分子SERS信号的分析得出了纳米银粒子在玻璃表面上可能的组装方式。以结晶紫(Crystal Violet)和孔雀石绿(Malachite Green)作为探测分子,采用便携式拉曼光谱仪测得两种分子不同浓度下的SERS谱。发现该方法所制得的纳米银膜有很好的表面增强效果。最后分析了半胱氨酸分子SERS信号对探针分子光谱的影响。
In the DC 10V voltage electrolysis polyvinyl alcohol and silver nitrate mixture of 3h preparation of nano silver glue, will be cysteine modified glass slides immersed in silver glue 24h obtained nano-silver film. The UV-visible spectrophotometer was used to observe the silver glue. Because of its narrow absorption at half height and width, the size distribution of the silver nanoparticles in the silver glue was relatively uniform. Meanwhile, scanning electron microscopy was used to characterize the silver film. Through the analysis of SERS signals of cysteine molecules, the possible ways of assembly of silver nanoparticles on the glass surface were obtained. Crystalline violet (Violet) and Malachite Green (Malachite Green) were used as the detection molecules, and the SERS spectra of the two molecules at different concentrations were measured by portable Raman spectrometer. It is found that the nano silver film obtained by the method has a good surface enhancement effect. Finally, the influence of SERS signal of cysteine molecule on the spectrum of probe molecule was analyzed.