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[目的]了解基质金属蛋白酶9(MMP9)及其组织抑制因子1(TIMP1)与煤工尘肺发生的关系。[方法]用酶联免疫吸附试验(ELISA)检测对照组、接尘组及病例组血清中MMP9、TIMP1的含量,计算相应的MMP9/TIMP1值。采用多因素回归对年龄和接尘工龄校正后进行分析。[结果]与对照组比较,接尘组血清中MMP0、TIMP1均有增加,尤其是MMP9增加较为明显(P<0.05),而TIMP1的差异没有统计学意义(P>0.05),各期煤工尘肺患者的MMP9、TIMP1浓度呈增加趋势,而MMP9/TIMP1值呈下降趋势,且与对照组相比,差异有统计学意义(P<0.05);经接尘工龄校正,与接尘组比较,Ⅰ期煤工尘肺的TIMP1升高(χ~2=8.80)以及Ⅲ期的MMP9、TIMP1升高(χ~2=12.18,8.20)。MMP9/TIMP1随煤工尘肺患者的晋期增加呈下降趋势(χ~2=13.42),差异均有统计学意义(P<0.05)。[结论]MMP9和TIMP1的平衡可能与煤工尘肺的发生与发展有关。
[Objective] To investigate the relationship between matrix metalloproteinase 9 (MMP9) and tissue inhibitor of metalloproteinase 1 (TIMP1) and the development of coal workers’ pneumoconiosis. [Methods] Serum levels of MMP9 and TIMP1 in control group, dust collection group and case group were detected by enzyme linked immunosorbent assay (ELISA), and the corresponding MMP9 / TIMP1 values were calculated. Multivariate regression was used to analyze the age and the occupational age of dust pick-up. [Results] Compared with the control group, serum MMP0 and TIMP1 in dust-exposed group increased, especially MMP9 increased more significantly (P <0.05), but the difference of TIMP1 was not statistically significant (P> 0.05) The levels of MMP9 and TIMP1 in pneumoconiosis patients tended to increase, while the levels of MMP9 / TIMP1 tended to decrease. Compared with the control group, the differences were statistically significant (P <0.05) TIMP1 (χ ~ 2 = 8.80) and stage Ⅲ MMP9, TIMP1 increased (χ ~ 2 = 12.18, 8.20) in phase Ⅰ coal workers’ pneumoconiosis. MMP9 / TIMP1 decreased with the increasing of Jin workers in coal workers with pneumoconiosis (χ ~ 2 = 13.42), the differences were statistically significant (P <0.05). [Conclusion] The balance of MMP9 and TIMP1 may be related to the occurrence and development of coal workers’ pneumoconiosis.