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采用回流法提取不同生长时期的桑叶,用HPLC法对其中的总黄酮含量进行测定分析,找出不同生长期桑叶总黄酮含量的变化规律,以便更为有效地利用不同时期的桑叶。研究结果显示,不同时期桑叶的总黄酮含量差异极大;桑树生长前期的桑叶总黄酮含量较低,桑树停止生长后到晚秋后的这段时间桑叶中总黄酮含量较高。
The mulberry leaves at different growth stages were extracted by reflux method. The content of total flavonoids in the mulberry leaves was determined by HPLC. The variation of total flavonoids content in mulberry leaves at different growth stages was found out to make more effective use of mulberry leaves at different growth stages. The results showed that there were significant differences in the content of total flavonoids in mulberry leaves in different periods. The content of total flavonoids in mulberry leaves was low at the early mulberry growth stage, and the content of total flavonoids in mulberry leaves was higher after the mulberry tree was stopped growing and after the late autumn.