３０对不育夫妇，在预计排卵日性交后４～１０小时，检查宫颈粘液中精子活力和形态，并与精液中对应参数比较，以探讨ＰＣＴ的方法、宫颈粘液对精子的选择机理及影响ＰＣＴ的因素。结果表明，粘液中精子活力和正常形态较精液中对应参数有显著提高，提示宫颈粘液通过阻止活力差的精子从而选择活力强、形态正常精子进入宫腔。文章认为限制、影响ＰＣＴ的因素众多，主要包括精液和宫颈粘液质量、ＰＣＴ时间及取样镜检时间等。并强调了掌握好试验方法的重要性。 30 pairs of infertile couples, 4 to 10 hours after the expected ovulation day of sexual intercourse, check the sperm motility and morphology of cervical mucus and compared with the corresponding parameters in semen to explore the PCT method of cervical mucus on sperm selection mechanism and affect the PCT the elements of. The results showed that the sperm motility and normal morphology in mucus compared with the corresponding parameters in seminal fluid were significantly increased, suggesting that cervical mucus through the inhibition of poor activity of sperm to select a strong vitality, morphologically normal sperm into the uterine cavity. The article holds that there are many factors affecting the PCT, including the quality of semen and cervical mucus, PCT time and sampling microscopic examination time. And stressed the importance of mastering the test method.