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目的探讨SEE患者体内的氧化/抗氧化状态及其与SLE疾病活动的关系。方法采用病例对照设计,应用分光光度法测定血浆氧化/抗氧化指标,ELISA法测定血浆抗核抗体(ANA)和抗dsDNA抗体滴度。应用两样本t检验,比较SLE组与正常对照组间氧化/抗氧化指标,采用Pearson相关分析比较机体氧化/抗氧化指标与疾病活动的临床/实验室指标间相关性。结果共检测SLE患者58例,正常人对照52例。SLE组血浆丙二醛浓度[(4.88±3.28)nmol/mL]高于正常人对照组[(2.96±1.90)nmol/mL](t= 4.70,P<0.01),总抗氧化能力[(14.06±5.65)U/mL]高于正常人对照组[(10.78±2.75)U/mL](t=3.59,P<0.001),超氧化物歧化酶活力[(57.19±19.40)U/mL]低于正常人对照组[(76.76±14.33)U/mL](t= -5.70,P<0.001)。SLE患者ANA与抗dsDNA抗体(r=0.800,P<0.01)、总抗氧化能力(r=0.30.P<0.05)、SLEDAI(r=0.38,P<0.05)间呈显著正相关。总抗氧化能力与抗dsDNA抗体(r=0.22.P<0.05)、血浆丙二醛(r=0.22,P<0.05)间呈显著正相关。结论SLE患者外周血氧化程度较正常人对照组高,氧化/抗氧化指标与SLE患者疾病活动情况呈显著相关。
Objective To investigate the oxidative / anti-oxidative status in SEE and its relationship with SLE disease activity. Methods A case-control study was designed. The plasma oxidation / antioxidant indexes were measured by spectrophotometry. The titer of plasma anti-nuclear antibody (ANA) and anti-dsDNA antibody was measured by ELISA. Two-sample t-test was used to compare the oxidant / antioxidant index between SLE group and normal control group. Pearson correlation analysis was used to compare the correlation between the body’s oxidation / antioxidant index and the clinical / laboratory index of disease activity. Results SLE patients were detected in 58 cases, 52 cases of normal controls. The plasma malondialdehyde concentration in SLE group was significantly higher than that in normal control group [(4.88 ± 3.28) nmol / mL [(2.96 ± 1.90) nmol / mL] (t = 4.70, P <0.01) ± 5.65) U / mL] was higher than that of the normal control group [(10.78 ± 2.75) U / mL] (t = 3.59, P <0.001) and the activity of superoxide dismutase [(57.19 ± 19.40) U / mL] In the normal control group [(76.76 ± 14.33) U / mL] (t = -5.70, P <0.001). There was a significant positive correlation between ANA and anti-dsDNA antibody (r = 0.800, P <0.01), total antioxidant capacity (r = 0.30, P <0.05) and SLEDAI (r = 0.38, P <0.05) in patients with SLE. Total antioxidant capacity was positively correlated with anti-dsDNA antibody (r = 0.22, P <0.05) and plasma malondialdehyde (r = 0.22, P <0.05) Conclusion The levels of peripheral blood oxidation in patients with SLE are higher than those in normal controls, and there is a significant correlation between oxidative / anti-oxidative indexes and disease activity in patients with SLE.