目的检测Wnt11及其受体Fzd5在成年肌萎缩脊髓侧索硬化症(ALS)转基因模型鼠脊髓内的表达变化,探讨Wnt11及其受体Fzd5在ALS发病中的作用。方法选取ALS转基因鼠和同窝野生型鼠各30只,分别于鼠龄95 d、108d、122 d取材,部分鼠经4%多聚甲醛心脏灌注固定、分离脊髓、冰冻切片,应用免疫荧光技术,检测脊髓中Wnt11及其受体Fzd5的表达变化;部分鼠取出脊髓、匀浆,应用RT-PCR技术检测不同时间点Wnt11及其受体Fzd5 mRNA的表达变化。结果免疫荧光结果显示,与同窝野生型鼠比较,95 d、108 d和122 d龄ALS转基因鼠脊髓中Wnt11阳性细胞明显增多。免疫荧光双标结果显示,Wnt11标记的阳性细胞与GFAP和β-tubulinⅢ共表达,ALS转基因鼠Wnt11/GFAP双阳性细胞增多。与同窝野生型鼠比较,ALS转基因鼠Wnt11 mRNA水平在95 d、108 d和122 d均升高。Wnt11受体Fzd5表达情况与Wnt11一致。结论 Wnt11和Fzd5在ALS转基因鼠脊髓中表达增多,表明Wnt11及其受体Fzd5表达升高与肌萎缩脊髓侧索硬化症密切相关。 Objective To detect the expression of Wnt11 and its receptor Fzd5 in the spinal cord of adult ALS transgenic mice and to explore the role of Wnt11 and its receptor Fzd5 in the pathogenesis of ALS. Methods Thirty (30) ALS transgenic mice and wild-type littermates were randomly divided into three groups: mice of 95 d, 108 d and 122 d respectively. Some of them were fixed by 4% paraformaldehyde in the heart. Spinal cord and frozen section were isolated. Immunofluorescence The expression of Wnt11 and its receptor Fzd5 in the spinal cord was detected. Some of the rats were removed from the spinal cord and homogenized. RT-PCR was used to detect the expression of Wnt11 and its receptor Fzd5 mRNA at different time points. Results The results of immunofluorescence showed that Wnt11 positive cells in the spinal cord of 95 days, 108 days and 122 days old ALS transgenic mice were significantly increased compared with wild-type littermates. Immunofluorescence double-labeled results showed that, Wnt11-positive cells were co-expressed with GFAP and β-tubulin Ⅲ, ALS transgenic mice Wnt11 / GFAP double positive cells increased. Compared with wild-type littermates, the Wnt11 mRNA level of ALS transgenic mice increased at 95 d and 108 d and 122 d respectively. Wnt11 receptor Fzd5 expression consistent with Wnt11. Conclusion The expression of Wnt11 and Fzd5 in the spinal cord of ALS transgenic mice increased, indicating that Wnt11 and its receptor Fzd5 expression is closely related to amyotrophic lateral sclerosis.