目的　探讨痢疾杆菌及其L型内毒素与细胞凋亡的关系。方法　将痢疾杆菌诱导为稳定L型 ,分别制备原菌内毒素和L型内毒素 ,用尾静脉注射法感染小鼠 ,观察小鼠的发病情况 ,并对死亡小鼠进行组织学及凋亡细胞检查。本实验共分三组 :A组原菌内毒素组 ,B组L型内毒素组 ,C组生理盐水组。结果　A组致小鼠细胞凋亡作用最强 ,以肝细胞凋亡最明显 ,A、B两组之间致凋亡的差异有显著性 (P <0 0 1)。结论　痢疾杆菌内毒素可致小鼠肝细胞凋亡 ,而其L型 ,由于失去细胞壁 ,内毒素丢失 ,致肝细胞凋亡能力大大降低 Objective To investigate the relationship between Shigella dysenteriae and its L-type endotoxin and apoptosis. Methods Shigella dysenteriae was induced to stable L-form, and the bacterial endotoxin and L-type endotoxin were respectively prepared. The mice were infected by tail vein injection, the incidence of mice was observed, and the histological and apoptotic cells an examination. The experiment is divided into three groups: group A bacterial endotoxin group, group B L-type endotoxin group, group C saline group. Results The apoptotic effect was the strongest in group A and the most obvious was in hepatocyte apoptosis. There was a significant difference in apoptosis between group A and group B (P <0.01). Conclusion Shigella endotoxin can induce hepatocyte apoptosis in mice, and its L-type, due to the loss of cell wall, the loss of endotoxin, greatly reduced the ability of liver cells to apoptosis