目的:采用微柱滤过法测定橄榄苦苷脂质体的包封率。方法:用20 ml的注射器装填葡聚糖凝胶,取40μl的样品加至柱头后,先用2倍柱体积的纯化水冲柱,再用50%的乙醇溶液洗柱,收集洗脱液,测定吸光度值,根据洗脱曲线计算脂质体中游离橄榄苦苷的含量,推算包封率。结果:在橄榄苦苷出峰处,脂质体对测定无干扰;橄榄苦苷在1~16μg·ml-1范围内,吸光度与浓度线性关系良好(r2=0.999 6);1,1.5及2 mg·ml-1橄榄苦苷与空白脂质体混合后的过柱回收率分别为(110.00±2.35)%,(109.75±2.63)%及(97.97±1.82)%;同一批橄榄苦苷脂质体重复测定6次包封率的RSD为1.76%。结论:本方法准确、简便,适于橄榄苦苷脂质体包封率的测定。 OBJECTIVE: To determine the entrapment efficiency of oleuropein liposomes by microcolumn filtration. METHODS: Dextran gel was loaded on a 20 ml syringe. After 40 μl of sample was added to the stigma, the column was washed with 2 column volumes of purified water and the column was washed with 50% ethanol. The eluate was collected, The absorbance value was determined. The content of free oleuropein in the liposome was calculated according to the elution curve, and the entrapment efficiency was calculated. Results: In the peak of the oleuropein, the liposomes had no interference with the assay. In the range of 1 ~ 16μg · ml-1, there was a good linear relationship between the absorbance and the concentration (r2 = 0.999 6); 1, 1.5 and 2 (110.00 ± 2.35)%, (109.75 ± 2.63)% and (97.97 ± 1.82)%, respectively. The same batch of oleuropein and liposome The RSD of 6th entrapment efficiency was 1.76%. Conclusion: This method is accurate and simple, suitable for the determination of encapsulation efficiency of oleuropein liposomes.