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目的:探讨βCGmRNA在肾细胞癌(RCC)和良性肾脏疾病组织中的表达情况。方法:采用RT-PCR并结合限制性内切酶法检测44例RCC和24例良性肾脏疾病组织βhCG基因及其亚型的表达。结果:RCC的βhCGmRNA阳性表达率为52％,晚期和低分化RCC的阳性表达率较高,但无统计学意义。良性肾脏疾病组织的βhCGmRNA阳性表达率为54％,包括3例多囊肾(3/6),7例肾萎缩(7/13),2例嗜酸性细胞瘤,1例肾盂肾炎。β7基因是RCC和良性肾脏疾病组织中最常见的βhCGmRNA亚型。结论:βhCG基因可能在恶性肾肿瘤和良性肾脏疾病中发挥作用,其病理生理和临床意义值得进一步研究。
Objective: To investigate the expression of βCG mRNA in renal cell carcinoma (RCC) and benign renal disease tissues. Methods: The expression of βhCG gene and its subtypes in 44 cases of RCC and 24 cases of benign renal disease were detected by RT-PCR and restriction endonuclease assay. Results: The positive expression rate of βhCG mRNA in RCC was 52%, while the positive expression rate of RCC in advanced and poorly differentiated RCC was higher but not statistically significant. The positive rate of βhCG mRNA in benign kidney disease was 54%, including 3 cases of polycystic kidney disease (3/6), 7 cases of renal atrophy (7/13), 2 cases of eosinophocytoma and 1 case of pyelonephritis. The β7 gene is the most common βhCG mRNA subtype in RCC and benign kidney disease tissues. Conclusion: βhCG gene may play a role in malignant renal tumors and benign renal diseases, and its pathophysiology and clinical significance deserve further study.