目的　研究培坤胶囊对子宫的作用 ,探索其治疗痛经的机制。方法　常规方法制备离体大鼠子宫标本 ,用己烯雌酚和催产素制造小鼠痛经模型 ,观察培坤胶囊对子宫的作用。结果　培坤胶囊能明显抑制子宫收缩力(11.6 %～ 6 5 .7%)、收缩频率 (2 8.2 %～ 6 0 .2 %)和活动度 (36 .6 %～ 84.6 %) ,其IC50 分别为 0 .6 9g·L-1、0 .83g·L-1和 0 .2 3g·L-1。 2 .0 g·kg-1和 5 .0 g·kg-1的培坤胶囊能延长痛经小鼠的扭体潜伏期 ,减少扭体次数。结论　培坤胶囊能抑制子宫收缩 ,有抗痛经作用。 Objective To study the effect of Peikun capsule on uterus and explore its mechanism for treating dysmenorrhea. Methods The isolated rat uterus specimens were prepared by conventional methods. The mouse dysmenorrhea model was made with diethylstilbestrol and oxytocin, and the effect of Peikun capsule on the uterus was observed. Results Peikun capsules can significantly inhibit uterine contractility (11.6 % - 65.7%), contraction frequency (28.2% - 60.2%) and activity (36.6% - 84.6%), and their IC50 values It is 0.66 g·L-1, 0.83 g·L-1, and 0.23 g·L-1. Peikun capsules with 2.0 g·kg-1 and 5.0 g·kg-1 prolonged the writhing latency of dysmenorrhea mice and reduced writhing frequency. Conclusion Peikun Capsule can inhibit uterine contraction and have anti-dysmenorrhea effect.