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目的研究双歧杆菌在葡聚糖硫酸钠(DSS)诱导的急性溃疡性结肠炎小鼠模型中的作用。方法将80只BALB/C小鼠均分为8组,每组10只。除正常对照组、单纯0501菌株组、单纯cl22菌株组外,其他5组动物均给予5% DSS造模7 d。在造模开始前2 d,阴性对照组用0.9%氯化钠液灌肠、阳性对照组用柳氮磺胺吡啶(SASP)20 mg/ml灌肠、DSS+0501菌株组用1×10~9 CFU/ml 0501菌液灌肠、DSS+cl22菌株组用1×10~9 CFU/ml cl22液菌灌肠。9 d后处死动物取结肠标本,行H-E染色,观察镜下结肠病理变化,并用免疫组化染色、RT-PCR技术分析结肠黏膜中白细胞介素(IL)-10 mRNA及蛋白表达。结果DSS造模过程中给予双歧杆菌灌肠小鼠(0501菌株组和cl22菌株组)的结肠炎性反应程度较模型组加重,结肠黏膜IL-10表达减少。而单纯给予双歧杆菌灌肠小鼠(单纯0501菌株组和单纯cl22菌株组)未见结肠炎表现。结论双歧杆菌的某些菌株在结肠黏膜屏障功能受损情况下,可加重溃疡性结肠炎小鼠的结肠黏膜损伤。
Objective To study the role of bifidobacteria in dextran sodium sulfate (DSS) -induced acute ulcerative colitis mouse model. Methods 80 BALB / C mice were divided into 8 groups with 10 in each group. Except for the normal control group, the other 5 animals were given 5% DSS for 7 days, except 0501 strain group and cl22 strain group alone. At 2 days before model establishment, the negative control group was treated with 0.9% sodium chloride solution. The positive control group was treated with SASP 20 mg / ml. The DSS + 0501 strain group was treated with 1 × 10 9 CFU / ml 0501 bacteria enema, DSS + cl22 strain group with 1 × 10 ~ 9 CFU / ml cl22 liquid enema. After 9 days, the colon specimens were sacrificed and H-E staining was performed. The pathological changes of the colon were observed. The expression of interleukin-10 mRNA and protein in colonic mucosa were analyzed by immunohistochemical staining and RT-PCR. Results The degree of colonic inflammatory reaction in Bifidobacterium enema mice (strain 0501 and cl22) during DSS modeling was more severe than that of model group, and the expression of IL-10 in colonic mucosa was decreased. The bifidobacterium enema mice (pure 0501 strain group and pure cl22 strain group) showed no colitis. Conclusion Some strains of Bifidobacterium can aggravate colonic mucosal injury in ulcerative colitis mice under the condition of impaired colonic barrier function.