目的 :研究莫尼定对大鼠视神经夹伤模型视网膜神经节细胞的保护作用。方法 :实验用SD大鼠 2 0只随机分为用药组 8只和对照组 12只。所有大鼠右眼用 40 g微型视神经夹紧贴球后夹持视神经 60秒 ,左眼未做夹持。用药组于夹伤前1小时及夹伤后每日腹腔注射莫尼定 1mg/kg ,阴性对照组于夹伤前 1小时及夹伤后每日腹腔注射生理盐水 5ml/kg ,实验观察2 8天。实验结束前 4天双上丘注射 3 %荧光金逆行标记视网膜神经节细胞。做视网膜铺片 ,距离视乳头中心上下左右各2mm拍摄照片 ,使用CPAS图像分析软件做节细胞定量分析 ,节细胞存活率 =右眼节细胞密度 /左眼节细胞密度× 10 0。结果 :用药组、对照组节细胞存活率分别为 61 0 1%和 53 48% ,两者之间存在显著性差异 (P =0 .0 3 5)。结论 :在大鼠视神经夹伤模型中 ,莫尼定具有明显的视网膜节细胞保护作用 Objective: To study the protective effect of monitidine on retinal ganglion cells in rat model of optic nerve crush. Methods: Twenty SD rats were randomly divided into treatment group (n = 8) and control group (n = 12). All rats right eye with 40 g miniature optic nerve clamping the optic nerve clip after 60 seconds, the left eye without clamping. The rats in the medication group were injected intraperitoneally with 1 mg / kg of monatin daily one hour before the injury and 1 ml / kg of the normal saline intraperitoneally 1 hour before the injury and 2 ml day. Four days before the end of the experiment, double retrograde labeling of retinal ganglion cells with 3% fluorescent gold was performed on the upper hill. Retinas were made. Photographs of 2 mm above and below the center of the optic nerve were taken and quantitatively analyzed using CPAS image analysis software. Cell viability was defined as the density of right eye node cells / the density of left eye node cells × 10 0. Results: The viability of ganglion cells was 61 0 1% and 53 48% respectively in the drug-treated group and the control group, with significant difference (P = 0.0305). CONCLUSION: Monatin has a protective effect on retinal ganglion cells in rat optic nerve crush model