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目的探讨HPSE AS-ODN对人胰腺癌细胞HPSE基因、蛋白表达及体内、外侵袭力的抑制作用。方法用脂质体将HPSE AS-ODN转染Panc-1细胞,逆转录-聚合酶链反应(RT- PCR)和Western印迹法分别检测转染后HPSE mRNA和蛋白表达;Transwell法检测体外侵袭力。建立裸鼠皮下Panc-1肿瘤模型,按10 mg／kg体重瘤体内注射AS-ODN隔日1次×10。治疗结束后断颈处死裸鼠,剥瘤称重,计算抑瘤率。结果 AS-ODN组mRNA和蛋白表达受到明显抑制,体外侵袭细胞数(个／视野)为60．00±9．31,体内平均瘤重(g)为1．860±0．505;对照组和NS-ODN组体外侵袭细胞数分别为253．00±9.35和240．75±9．36,体内平均瘤重分别为2．948±0．483和2．768± 0．615。AS-ODN组与对照组相比差异有统计学意义(P<0．01)。结论 AS-ODN抑制人胰腺癌细胞 HPSE mRNA和蛋白的表达,并降低癌细胞的侵袭力。
Objective To investigate the inhibitory effect of HPSE AS-ODN on HPSE gene and protein expression and in vitro and in vivo invasiveness in human pancreatic cancer cells. Methods HPSE AS-ODN was transfected into Panc-1 cells by lipofectamine 2000. The expression of HPSE mRNA and protein was detected by reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting respectively. . A subcutaneous Panc-1 tumor model was established in nude mice. AS-ODN was injected into the body at 10 mg / kg body weight once a day for 10 days. The nude mice were sacrificed after the treatment, and the tumors were weighed and the tumor inhibition rate was calculated. Results The mRNA and protein expression of AS-ODN group was significantly inhibited. The number of invaded cells in vitro (a / field) was 60.00 ± 9.31 and the mean in vivo tumor weight (g) was 1.860 ± 0.505. In the control group and The number of invasive cells in NS-ODN group was 253.00 ± 9.35 and 240.75 ± 9.36, respectively. The average in vivo tumor weights were 2.948 ± 0.483 and 2.768 ± 0.615, respectively. The difference between AS-ODN group and control group was statistically significant (P <0.01). Conclusion AS-ODN can inhibit the expression of HPSE mRNA and protein in human pancreatic cancer cells and reduce the invasiveness of cancer cells.