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不同病圃同一套寄主的田间表现及其穗颈瘟分离菌株的遗传宗谱和致病性分析试验结果证明,不同生态稻区稻瘟病菌的小种分布类型不同,对品种的致病性也存在差异。将已经过RFLJ和PCR技术进行DNA指纹分析,并划分为不同遗传宗谱的稻瘟病菌株进行致病性测定。结果表明,菌株的致病性与其遗传宗谱类型、生理小种类型、寄主及采集地点存在密切的关系:(1)同一遗传宗谱、分离自相同寄主、鉴定出来的生理小种类型又较接近的菌株,其致病性相似率最高;(2)同一遗传宗谱的菌株,如果所鉴定的生理小种类型较接近,且来自同一采集地点,即使是不同寄主上分离的菌株,其致病性相似率也较高;(3)不同遗传宗谱、不同寄主上分离的菌株,致病性差异较大。目前,在选择应用于品种或资源抗性鉴定的菌株时,必须将鉴别寄主测定的小种致病型与菌株的宗谱类型、寄主及采集地点结合起来,才能保证选出的菌株可靠、代表性强。
The field performance of the same set of hosts in different nurseries and the genetic lineage and pathogenicity test results of isolates of panicle blast showed that the races of Magnaporthe grisea in different ecological rice regions have different types of races and different pathogenicity has a difference. DNA fingerprinting, which has been subjected to RFLJ and PCR techniques, was conducted to determine the pathogenicity of M. grisea strains divided into different genetic lineages. The results showed that the pathogenicity of the strains was closely related to their genetic lineage types, physiological races, hosts and collection sites: (1) the same genetic lineage was isolated from the same host and the identified races Close to the strains, the highest similarity of pathogenicity; (2) strains of the same genetic lineage, if the identified racer types are close and come from the same collection site, even strains isolated from different hosts, which caused The similarities of pathogenicity were also higher. (3) The strains isolated from different genealogies and hosts had different pathogenicity. At present, when selecting strains that are used for the identification of varieties or resource resistances, it is necessary to combine the pedigree type, the host and the collection site of the minor species and the host strains determined by the identification host to ensure that the selected strains are reliable and represent Strong.