在两种品系小鼠中建立、优化和规范B组3型柯萨奇病毒(CVB3)诱导的病毒性心肌炎小鼠模型。首先以100TCID50-10 000TCID50之间剂量的CVB3Nancy株病毒经腹腔感染易感BALB/c雄性小鼠,通过体重减轻率、死亡率和心脏病理及心肌CK-MB水平,评估建立最佳病毒性心肌炎的最佳剂量;而后摸索了在C57BL/6小鼠建立病毒性心肌炎的CVB3剂量。通过精细比较发现雄性BALB/c小鼠致心肌炎的最佳CVB3病毒滴度数量级在1000TCID50;进一步确定1500TCID50是在雄性BALB/c小鼠中诱导病毒性心肌炎的最佳剂量;C57BL/6小鼠不是易感小鼠,但在1500TCID50CVB3感染下也可诱导轻微可见的病毒性心肌炎,便于在多数基因敲除鼠内的病毒性心肌炎研究。本研究为规范病毒性心肌炎模型的建立、为后续病毒性心肌炎机制的深入研究奠定了重要的基础模型。 A mouse model of viral myocarditis induced by coxsackievirus 3 (CVB3) group B was established, optimized, and regulated in both strain mice. Susceptible BALB / c male mice were first infected with CVB3Nancy strain at a dose of 100 TCID50-10 000 TCID50, and the best viral myocarditis was evaluated by weight loss, mortality and cardiopathological and myocardial CK-MB levels The optimal dose was then explored to establish the dose of CVB3 for viral myocarditis in C57BL / 6 mice. The best CVB3 virus titer for male myocarditis caused by myocarditis in male BALB / c mice was found by fine comparison to be in the order of 1000 TCID50; 1500 TCID50 was further determined to be the optimal dose to induce viral myocarditis in male BALB / c mice; C57BL / 6 mice were not Susceptible mice, but also under the 1500TCID50CVB3 infection can also induce a slight visible viral myocarditis, facilitating the study of viral myocarditis in most knockout mice. This study to standardize the establishment of viral myocarditis model for the follow-up of viral myocarditis in-depth study has laid an important foundation model.