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在对中国柱花草炭疽病进行广泛调查和病原采样收集的基础上 ,利用RAPD分子标记技术对 43个代表性菌株进行了基因组DNA分析 ,并与 2 76份国外菌株进行了综合聚类分析。结果表明所用 8个引物的扩增片段位于 0 3～ 2 8kb之间 ,菌株间呈现显著的DNA多态性。以柱花草起源中心———南美的柱花草炭疽菌分类为基础 ,中国柱花草炭疽菌可划分成 3大类型即Ⅱ、Ⅲ、Ⅵ类。中国菌株与来自柱花草起源中心———南美的菌株相比之下 ,其生物多样性和遗传变异性则相对简单。就中国菌株而言海南菌株与广西、广东菌株相比多样性较丰富 ,中国柱花草胶孢炭疽菌正在出现种内遗传分化。从聚类结果看 ,通常来自于同一个地理区域或同一个寄主基因型的菌株聚成一类 ,即同一RAPD聚类组内的菌株通常来自于同一寄主基因型或同一地理区域。说明来自不同寄主基因型或物种的炭疽菌在遗传基因上具有专化性 ,而地理上隔离的国家或地区的柱花草炭疽病原菌各自具有相对独立的进化途径。
Based on extensive investigation of Colletotrichum anthracnose and collection of pathogenic samples in China, 43 representative strains were analyzed by genomic DNA using RAPD molecular markers, and a total of 276 foreign isolates were analyzed by cluster analysis. The results showed that the amplified fragments of eight primers used ranged from 0 to 28kb, showing significant DNA polymorphism among isolates. Based on the classification of Stylosanthes anthracnosis in South America, the center of Stylosanthes, anthracnose of Stylosanthes can be divided into three major types, namely Ⅱ, Ⅲ and Ⅵ. Compared with strains originating in the center of Stylosanthes - South America, the Chinese strain is relatively simple in its biological diversity and genetic variability. Hainan strains of Chinese strains and Guangxi, Guangdong, compared to the rich diversity of strains, Colletotrichum Colletotrichum Chinese species are emerging intraspecific genetic differentiation. From the clustering results, generally, strains from the same geographical area or the same host genotype are clustered, that is, the strains in the same RAPD cluster usually come from the same host genotype or the same geographical area. This indicates that anthrax species from different host genotypes or species are genetically specific, while the anthrax of Colletotrichum in geographically isolated countries and regions, respectively, has a relatively independent evolutionary path.