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目的:评价青藤碱对2,4,6-三硝基苯磺酸(TNBS)灌肠引起的Th1细胞介导的小鼠实验性结肠炎的治疗作用,并探讨其作用机制。方法:为评价青藤碱对结肠炎的治疗作用,将Balb/c小鼠随机分为乙醇对照组,TNBS模型组,青藤碱低(50mg·kg~(-1))、中(100 mg·kg~(-1))、高(200 mg·kg~(-1))剂量组,每组10只。为观察青藤碱对TNBS结肠炎的治疗作用,第1剂TNBS给药后7 d开始灌胃给予青藤碱,持续给药21 d。第28天处死动物,观察结肠黏膜的损伤程度,测定结肠髓过氧化物酶(MPO)活性,ELISA法分别测定结肠炎症细胞因子(TNF-α、IL~(-1)7和IL-23)蛋白水平。结果:与TNBS模型组比较,青藤碱中、高剂量组的体质量、大体损伤评分及组织学表现均显著改善(P<0.05),MPO酶活性显著降低(P<0.05),结肠黏膜中TNF-α、IL~(-1)7和IL-23的蛋白水平均较TNBS组下降(P<0.05)。结论:青藤碱对TNBS诱导的慢性小鼠结肠炎具有治疗作用,作用机制与青藤碱对Th1炎症细胞因子的抑制有关。
OBJECTIVE: To evaluate the therapeutic effect of sinomenine on Th1 cell-mediated experimental colitis induced by 2,4,6-trinitrobenzene sulfonic acid (TNBS) enema and its mechanism of action. Methods: To evaluate the therapeutic effect of sinomenine on colitis, Balb / c mice were randomly divided into ethanol control group, TNBS model group, sinomenine low (50 mg · kg -1), medium Kg ~ (-1)) and high dose (200 mg · kg ~ (-1)), with 10 rats in each group. In order to observe the therapeutic effect of Sinomenine on TNBS colitis, sinomenine was given intragastrically on the 7th day after the first dose of TNBS was administered for 21 days. The animals were sacrificed on the 28th day to observe the degree of colonic mucosal injury. The activity of colonic myeloperoxidase (MPO) was measured. The inflammatory cytokines (TNF-α, IL-7 and IL-23) Protein level. Results: Compared with TNBS model group, the body weight, gross injury score and histological score of sinomenine group were significantly improved (P <0.05), and the activity of MPO enzyme was significantly decreased (P <0.05) The protein levels of TNF-α, IL-7 and IL-23 were significantly lower than those of TNBS group (P <0.05). Conclusion: Sinomenine has a therapeutic effect on TNBS-induced chronic colitis in mice. Its mechanism is related to the inhibition of sinomenine on Th1 inflammatory cytokines.