【摘 要】
：
2008年6月从湖州某患病草鱼池塘采集草鱼出血病疑似病样,将除菌过滤后的患病鱼肝、脾、肾组织滤液,注射健康的8～10 cm的草鱼鱼种.5d后草鱼开始发病,且症状与原发病症状一样,死
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2008年6月从湖州某患病草鱼池塘采集草鱼出血病疑似病样,将除菌过滤后的患病鱼肝、脾、肾组织滤液,注射健康的8～10 cm的草鱼鱼种.5d后草鱼开始发病,且症状与原发病症状一样,死亡率为57%,对照组未有死亡.将无菌处理的病样滤液接种草鱼肾细胞(CIK),连续接5代均出现明显的细胞病变(CPE),并与草鱼呼肠孤病毒参考株所产生的CPE一致.该株病毒的TCID50为10-8/0.1ml.内脏组织经超薄切片,电子显微镜观察,发现组织内有大量的病毒颗粒,大小均一,近似球形,直径约70～75 nm.理化鉴定表明,氯仿、乙醚处理组病毒的感染力和对照组相比并没有多大变化,说明该株病毒对氯仿和乙醚有一定的抗性.经草鱼呼肠孤病毒特异性的RT-PCR检测,获得阳性的目的片段,测序的结果与草鱼呼肠孤病毒相应序列的同源性达99%以上.上述鉴定结果表明所分离的病毒为草鱼呼肠孤病毒,将其命名为HZ2008.
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