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箭筈豌豆是我国传统肥饲兼用的豆科绿肥作物,有关箭筈豌豆根瘤菌的研究尚属空白。为获得抗逆性强的根瘤菌菌株,在青海主产区采集样本,经分离、鉴定得到61株箭筈豌豆根瘤菌。采用水培回接法,通过主成分分析筛选出5株对宿主结瘤生长促进作用较强的根瘤菌(J1-3-2、J2-6-2、J3-12-1、J4-3-3和J5-5-3)。对该5株根瘤菌进行了离体耐盐性评价,发现菌株J5-5-3最大耐盐极限值在0.9%左右,其他4菌株最大耐盐极限值均在1.2%左右,并且5菌株的耐盐特征呈3个明显不同类型:J3-12-1和J2-6-2耐盐性最强,菌株J1-3-2和J4-3-3居中,菌株J5-5-3最差。进一步采用盆栽方法评价了其中3株(每类型1株)根瘤菌-箭筈豌豆共生体的耐盐性,结果表明,在0.15%NaCl胁迫下接种根瘤菌J5-5-3植株结瘤数最多,在0.30%NaCl胁迫下3种接种处理无显著差异;在0.15%和0.30%NaCl胁迫下,接种J3-12-1共生体株高显著大于对照(P<0.05);在0.15%和0.30%NaCl胁迫下根瘤菌J3-12-1和J5-5-3可显著提高植株干重(P<0.05);J5-5-3在0.15%和0.30%NaCl胁迫下可显著提高植株含氮量并显著提高0.15%NaCl胁迫下的植株吸氮量(P<0.05);J3-12-1显著提高了0.15%NaCl胁迫下的植株吸氮量(P<0.05)。综合来看,J3-12-1和J5-5-3可以作为在盐胁迫下提高箭筈豌豆生物量和含氮量的预选菌株。
Arrowheadia pea is a legume green manure crop used by both traditional fattening and feeding in China. The research on rhizobia of arrowhead pea is still blank. In order to obtain rhizobia strains with strong resistance, 61 samples of Rhizobium invade were isolated and identified in the main producing areas of Qinghai. Five strains of Rhizobium (J1-3-2, J2-6-2, J3-12-1, J4-3-J1-3-2) with strong promoting effect on host nodulation growth were screened by principal component analysis. 3 and J5-5-3). The in vitro salt tolerance of the five Rhizobia strains was evaluated and the maximum salt tolerance limit of strain J5-5-3 was found to be about 0.9%. The maximum salt tolerance limit of the other four strains was about 1.2% Salt tolerance characteristics showed three distinct types: J3-12-1 and J2-6-2 had the strongest salt tolerance, the strains J1-3-2 and J4-3-3 were the center, and the strain J5-5-3 was the worst. Pot experiments were conducted to evaluate the salt tolerance of rhizobium - arrowhead pea symbiosis in three of them (one for each type). The results showed that the number of nodulation strains inoculated with Rhizobia strain J5-5-3 under 0.15% NaCl stress was the highest , Respectively. There was no significant difference among the three inoculation treatments under the stress of 0.30% NaCl. The plant height of J3-12-1 inoculated with 0.15% and 0.30% NaCl was significantly higher than that in the control (P <0.05) Rhizobia J3-12-1 and J5-5-3 significantly increased plant dry weight under NaCl stress (P <0.05); J5-5-3 significantly increased plant nitrogen content under 0.15% and 0.30% NaCl stress The nitrogen uptake of plants under 0.15% NaCl stress was increased significantly (P <0.05). J3-12-1 significantly increased the uptake of nitrogen by 0.15% NaCl stress (P <0.05). Taken together, J3-12-1 and J5-5-3 can be used as pre-selected strains to increase the biomass and nitrogen content of arrowroot pea under salt stress.