为了对肠型点状产气单胞菌 5 8- 2 0 - 9菌株和鱼害粘球菌 G4 菌株的融合子进行选择鉴定 ,应用定性和定量药物敏感性试验 ,对两种菌原生质体融合的遗传标记进行了筛选 .结果表明 ,这两种细菌对红霉素和庆大霉素交叉耐药 .在此基础上 ,测定了红霉素和庆大霉素在选择培养基中的适用浓度 .筛选出肠型点状产气单胞菌 5 8- 2 0 - 9菌株的遗传标记为 Erm(红霉素抗性 ) ,鱼害粘球菌 G4 菌株的遗传标记为 Grm(庆大霉素抗性 ) In order to select and identify the fusants of Enterobacter aerogenes 5 8- 2 0-9 strain and Mycoides mycoides G4 strain, qualitative and quantitative drug susceptibility tests were conducted to fuse the two strains of protoplasts The results showed that the two bacteria were resistant to erythromycin and gentamycin.On the basis of this, the suitable concentrations of erythromycin and gentamicin in the selection medium were determined. The selected genetic markers of Erysipengomyces aeruginosa 5 8- 20-9 strains were Erm (erythromycin resistance) and the genetic markers of Mycoides megasus G4 strain were Grm (gentamycin resistance )