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采用激光共聚焦扫描显微镜，观察黄嘌呤／黄嘌呤氧化酶反应系统（ Ｘ／ＸＯ）生成不同浓度的超氧阴离子自由基（Ｏ２）致培养的大鼠肝卵圆细胞（ＷＢ细胞）胞质内钙浓度的变化，结果发现：仅小剂量的Ｏ２（Ｘ：２００ｍｏｌ／Ｌ ＸＯ：０．２ｍｕ／ｍＬ）引起胞质内钙浓度升高，约３０ｓ后达到峰值，６０ｓ后恢复正常。部分细胞观察到数次钙浓度间断升高、幅度逐渐下降的现象。超氧化物歧化酶（ ＳＯＤ）可抑制此变化，过氧化氢酶（ ＣＡＴ）无效果。细胞在无钙液中观察仍出现钙峰，但受内钙库耗竭剂Ｔｈａｐｓｉｇａｒｇｉｎ阻断。结果表明，小剂量Ｏ２可诱使肝卵圆胞胞内钙库钙释放造成瞬间胞质自由钙浓度增高，并可能引发胞内钙浓度的衰减振荡，此可能与Ｏ２通过影响胞内重要信号转导因子钙，调控细胞生物学功能如增殖、转化相关。
The confocal laser scanning microscopy was used to observe the xanthine / xanthine oxidase reaction system (X / XO) to generate different concentrations of superoxide anion radical (O2) in cultured rat hepatic oval cells (WB cells) within the cytoplasm The results showed that only a small dose of O2 (X: 200mol / L XO: 0.2mu / mL) caused intracellular Ca2 + concentration to rise, reaching a peak after about 30s and returned to normal after 60s. Some cells observed intermittent increase in calcium concentration several times, the gradual decline in the phenomenon of amplitude. Superoxide dismutase (SOD) can inhibit this change, catalase (CAT) no effect. Cells still observed calcium peaks in calcium-free fluids, but were blocked by Thapsigargin, an endosomal calcium depletion agent. The results showed that low dose of O2 could induce intracellular Ca2 + release from hepatic oval cells, which resulted in the increase of free Ca2 + concentration in cytoplasm and the decrease of intracellular Ca2 + concentration. This may be related to the effect of O2 on intracellular Ca2 + Ca factor, regulation of cell biological functions such as proliferation, transformation related.