目的　研究不同浓度利多卡因对中枢神经系统海马锥体神经元电压依赖性钠通道的影响 ,明确利多卡因对神经元的保护和损伤与电压依赖性钠通道的关系。方法　应用全细胞膜片钳方法记录不同浓度利多卡因 (10 -5mol/L～ 10 -2 mol/L)时大鼠急性分散海马锥体神经元电压依赖性钠通道电流的变化。结果　利多卡因对钠通道阈电位影响不大 ,以浓度依赖的方式抑制电压依赖性钠电流 ,随利多卡因浓度的增加其抑制钠电流的作用加强 ,EC50 为 (0 39± 0 0 5 )mmol/L。结论　利多卡因的脑保护作用与其抑制电压依赖性的钠通道的作用有关 ,其毒性作用与电压依赖性的钠通道无关 Objective To investigate the effects of different concentrations of lidocaine on the voltage-dependent sodium channel in the pyramidal neurons of the central nervous system in hippocampus and to clarify the relationship between the protection and injury of neurons and the voltage-dependent sodium channel in lidocaine. Methods The changes of voltage-dependent sodium currents in acutely distributed hippocampal pyramidal neurons of rats were recorded by whole-cell patch clamp method with different concentrations of lidocaine (10 -5 mol / L ~ 10 -2 mol / L). Results Lidocaine had little effect on the threshold voltage of sodium channel and inhibited voltage-dependent sodium current in a concentration-dependent manner. The effect of lidocaine on sodium current was enhanced with the increase of lidocaine concentration. The EC50 was (0 39 ± 0 0 5) mmol / L. Conclusion The protective effect of lidocaine on the brain is related to its inhibitory effect on voltage-dependent sodium channel, and its toxic effect is independent of voltage-dependent sodium channel