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以玉米遗传连锁图谱IBM2 2008 Neighbors为参考图谱,整合65个抗玉米灰斑病QTL,构建QTL综合图谱。采用元分析方法优化65个QTL,获得11个“一致性”QTL区间,分别位于染色体bin区的1.05、1.06、2.03、2.07、3.02、4.05、5.03、5.05、7.02、8.07、9.03位置,其在遗传连锁图谱上对应的位置分别为442.21、528.27、228.10、478.00、74.65、311.59、169.62、302.35、252.19、422.70、257.93 cM。对两个具有较多报道和较高表型贡献的“一致性”QTL区间bin1.05和bin1.06,从MaizeGDB网站搜索得到324个基因。因抗病基因在结构上具有高度保守性,将324个基因分别与水稻和拟南芥基因组进行同源比对,在bin1.05和bin1.06内分别确定了7个和3个基因作为玉米抗灰斑病候选基因。
Based on the maize genetic linkage map IBM2 2008 Neighbors as a reference map, 65 QTLs for resistance to Cercospora zea were integrated to construct a QTL integrated map. Sixteen QTLs were optimized by using meta-analysis, and 11 QTLs for “consistency” were obtained, which were located at 1.05,1.06,2.03,2.07,3.02,4.05,5.03,5.05,7.02,8.07,9.03, respectively, The corresponding positions on the genetic linkage map were 442.21, 528.27, 228.10, 478.00, 74.65, 311.59, 169.62, 302.35, 252.19, 422.70, 257.93 cM, respectively. Two of the “consistent” QTL intervals bin1.05 and bin1.06 with more reports and higher phenotype contributions were searched from the MaizeGDB website for 324 genes. 324 genes were homologously aligned with the genomes of rice and Arabidopsis, respectively, and 7 and 3 genes were identified as maize in bin1.05 and bin1.06, respectively Gray rust resistant candidate gene.