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目的研究过氧化物酶体增殖物激活受体δ基因PPARD-87位点多态性与中国人糖尿病及代谢综合征的相关性。方法选取663例上海地区中国人(其中糖耐量正常者376名,新诊断糖尿病患者287例),用PCRRFLP检测PPARD基因-87T→C变异,并检测所选人群的临床指标。结果PPARD基因-87T→C多态与新诊断糖尿病患者中代谢综合征相关(P<0.05)。在糖尿病患者中,此多态与肥胖相关(P<0.05);在正常糖耐量人群中,此多态与血脂紊乱发生相关(P<0.01)。此外,在正常糖耐量人群(P<0.01)及糖尿病患者(P<0.05)中,C等位基因在胰岛素抵抗亚组频率高相关。Logistic回归分析证实,在中国人群中PPARD基因-87T→C变异是正常糖耐量人群中血脂紊乱(OR=0.498,P<0.01)及糖尿病患者肥胖(OR=0.465,P<0.01)的独立危险因子。结论PPARD基因-87T→C变异与代谢综合征相关。
Objective To investigate the association of PPARD-87 polymorphism of peroxisome proliferator activated receptor δ gene with Chinese diabetes mellitus and metabolic syndrome. Methods A total of 663 Chinese patients (376 with normal glucose tolerance and 287 with newly diagnosed diabetes) were enrolled in this study. PCR-RFLP was used to detect the -87T → C mutation in PPARD gene and the clinical parameters of the selected population were determined. Results PPARD gene-87T → C polymorphism was associated with metabolic syndrome in newly diagnosed diabetic patients (P <0.05). In diabetic patients, this polymorphism was associated with obesity (P <0.05). In normal glucose tolerance subjects, this polymorphism was associated with dyslipidemia (P <0.01). In addition, the C allele was highly correlated with the frequency of insulin resistance subgroups in normal glucose tolerance (P <0.01) and diabetic patients (P <0.05). Logistic regression analysis confirmed that the -87T → C mutation of PPARD gene in Chinese population was an independent risk factor for dyslipidemia (OR = 0.498, P <0.01) and obesity in diabetic patients (OR = 0.465, P <0.01) . Conclusion PPARD gene-87T → C variation is associated with metabolic syndrome.