论文部分内容阅读
目的探讨罗格列酮(ROSI)对脂多糖(LPS)诱导的多器官功能障碍综合征(MODS)大鼠器官功能损伤的保护作用。方法健康雄性SD大鼠40只,随机分为四组(每组10只):①正常对照组;②LPS刺激组;③罗格列酮(ROSI)预处理组;④2-氯-5-硝基苯胺(GW9662)预处理组。在每组操作结束后4h取血用于主要器官生化指标的检测:肝功能、肾功能、心脏功能、胰腺功能。结果主要器官的生化指标在LPS组明显升高,与正常对照组比较差异有统计学意义(P<0.01)。ROSI预处理则大大减轻了指标的升高,与LPS组比较差异有统计学意义(P<0.01)。GW9662预处理则逆转了这种保护作用,器官功能指标低于LPS组,与LPS组比较差异无统计学意义(P>0.05)。结论罗格列酮预处理可减轻MODS大鼠器官功能损伤,为过氧化物酶体增殖物激活受体-γ(PPAR-γ)激动剂用于临床防治MODS提供了新的思路和依据。
Objective To investigate the protective effect of rosiglitazone (ROSI) on organ dysfunction induced by lipopolysaccharide (LPS) in multiple organ dysfunction syndrome (MODS) rats. Methods Forty healthy male SD rats were randomly divided into four groups (n = 10): ① normal control group; ② LPS stimulation group; ③ Rosiglitazone (ROSI) pretreatment group; ② 2-chloro-5-nitro Aniline (GW9662) pretreatment group. Blood samples were taken at 4h after the end of each group for the detection of biochemical markers of major organs: liver function, renal function, cardiac function, and pancreatic function. Results The biochemical parameters of major organs were significantly increased in LPS group compared with those in normal control group (P <0.01). ROSI pretreatment significantly reduced the index, which was significantly different from LPS group (P <0.01). GW9662 preconditioning reversed this protective effect, and the indexes of organ function were lower than LPS group, but no significant difference compared with LPS group (P> 0.05). Conclusion Rosiglitazone preconditioning can reduce organ dysfunction in MODS rats and provide new ideas and basis for the prevention and treatment of MODS by PPAR-γ agonist.