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目的:研究人参总皂苷联合淫羊藿苷对血管性痴呆大鼠学习记忆、氧自由基及海马CA1区神经细胞凋亡的影响。方法:采用反复夹闭双侧颈总动脉再灌注同时腹腔注射硝普钠方法建立血管性痴呆大鼠模型,连续给药21d后,用八臂电迷宫法判断给药前后大鼠的学习和记忆能力,再进行脑组织SOD、MDA的测定和海马CA1区神经细胞凋亡检测。结果:与假手术组大鼠相比,模型大鼠电迷宫学习和记忆错误次数明显增加(P<0.05),大鼠造模前后连续灌胃给药21 d后,大鼠上述行为学指标得到明显改善(P<0.05),脑组织SOD活性提高(P<0.05)和MDA含量降低(P<0.01),海马CA1区神经细胞凋亡数减少(P<0.05)。结论:人参总皂苷联用淫羊藿苷对血管性痴呆大鼠学习记忆障碍有显著的预防和治疗作用,可能与通过改善血管性痴呆大鼠大脑自由基代谢和海马CA1区神经细胞损伤有关,从而起到保护大脑的作用。
Objective: To study the effects of ginsenosides combined with icariin on learning and memory, oxygen free radicals and neuronal apoptosis in hippocampal CA1 area of rats with vascular dementia. Methods: The model of vascular dementia was established by repeated occlusion of bilateral carotid artery reperfusion and intraperitoneal injection of sodium nitroprusside. After 21 days of continuous administration, the learning and memory of rats Ability, and then the determination of brain SOD, MDA and neuronal apoptosis in the hippocampal CA1 area. Results: Compared with the sham operation group, the number of maze learning and memory errors in the model rats increased significantly (P <0.05). After the rats were given intragastric administration for 21 days, the above behavioral indexes were obtained (P <0.05), the activity of SOD in brain tissue increased (P <0.05) and the content of MDA decreased (P <0.01). The apoptosis of hippocampal CA1 area decreased (P <0.05). Conclusion: Ginseng total saponins combined with icariin have significant preventive and therapeutic effects on learning and memory impairment in vascular dementia rats, which may be related to the improvement of free radical metabolism and damage of hippocampal CA1 neurons in rats with vascular dementia. Thus playing a role in protecting the brain.