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采用AFLP分子标记技术 ,对我国不同纬度的 2 0份野生大豆 (G .soja)和栽培大豆 (G .max)进行了多样性分析 ,结果表明 :( 1)我国野生大豆的遗传多样性较栽培大豆更为丰富 ;( 2 )根据AFLP分析结果 ,将野生大豆和栽培大豆完全划分为 2类 ,并发现野生大豆和栽培大豆的种特异谱带 ,说明野生大豆和栽培大豆作为 2个种是有遗传基础的 ;( 3)野生大豆和栽培大豆不同纬度品种间纬度相近的首先聚在一起 ,表明不同进化类型的大豆其遗传距离与纬度可能存在一定的相关性。本研究的结果为大豆分类、进化、起源等研究提供一定的证据 ,同时也表明AFLP标记技术可以用于大豆分类及遗传多样性研究
AFLP molecular markers were used to analyze the diversity of 20 wild G. soja and G. max cultivars at different latitudes in China. The results showed that: (1) The genetic diversity of wild soybean in China was higher than that of cultivated soybean Soybean is more abundant; (2) Based on the results of AFLP analysis, wild soybean and cultivated soybean are completely divided into two categories, and the species-specific bands of wild soybean and cultivated soybean are found, indicating that wild soybean and cultivated soybean are two species (3) The similar latitudes between wild soybean and cultivated soybean were clustered together firstly, which indicated that there may be some correlation between the genetic distance and latitude of different evolutionary types of soybean. The results of this study provide some evidence for the research on the classification, evolution and origin of soybean, and also indicate that the AFLP marker technology can be applied to the classification of soybean and genetic diversity