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目的：探讨 MTT法应用于肿瘤细胞化疗敏感性检测的意义 ,及化疗与肿瘤浸润淋巴细胞 (tumor infiltrating lymphocyte,TIL)过继免疫治疗的合理应用。方法：应用 MTT法检测肿瘤细胞对化疗药物的敏感性和化疗对 TIL的毒性。结果：肿瘤细胞和 TIL细胞数都与生成的甲 ? (formazan)的光密度 (OD)值呈线性正相关。肿瘤细胞对化疗药物的敏感性存在较大个体差异。 EADM与 DDP， DDP与 MMC联合化疗存在协同作用， EADM与 5 FU联合化疗存在拮抗作用。化疗药物对 TIL的毒性作用大于对肿瘤细胞的杀伤作用。结论： MTT法应用于肿瘤细胞化疗敏感性检测以指导临床个体化治疗，具有筛选敏感性化疗药物和避免非敏感性化疗对机体抗肿瘤免疫细胞毒性的双重积极意义。 TIL过继免疫治疗与化疗不宜同时应用。
Objective: To explore the significance of MTT assay in the chemosensitivity of tumor cells and the rational application of chemotherapy and tumor infiltrating lymphocyte (TIL) adoptive immunotherapy. Methods: The sensitivity of tumor cells to chemotherapeutic drugs and the toxicity of chemotherapy on TIL were detected by MTT assay. RESULTS: The tumor cells and the number of TIL cells were linearly positively correlated with the optical density (OD) of the formed formazan (formazan). There is a large individual difference in the sensitivity of tumor cells to chemotherapy drugs. There was a synergistic effect between EADM and DDP, DDP and MMC combined chemotherapy, and EADM and 5-FU combined chemotherapy had antagonistic effects. The toxicity of chemotherapeutic drugs to TIL is greater than the killing effect on tumor cells. Conclusion: The MTT method is applied to the detection of tumor cell chemosensitivity to guide individualized clinical therapy. It has the dual positive significance of screening sensitive chemotherapy drugs and avoiding the anti-tumor immune cytotoxicity of non-sensitive chemotherapy. TIL adoptive immunotherapy and chemotherapy should not be applied simultaneously.