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目的研究螺内酯(spironolactone,SPT)对离体血管平滑肌张力影响,同时探讨SPT的作用机制。方法离体血管平滑肌张力记录法,应用SPT,观测其对Sprague Dawley(SD)大鼠离体胸主动脉环的作用及不同工具药的影响。结果 SPT对KCl(30 mmol.L-1)和NE(1μmol.L-1)预收缩的血管环具有浓度依赖的舒张作用,对内皮完整和去内皮血管环舒张作用无差异,该舒张作用为非内皮依赖性。Na+/H+交换阻滞剂氨氯吡咪(amiloride,AM,1μmol.L-1)对SPT的舒血管作用有抑制作用。在KCl预收缩基础上,加入钾通道阻断剂氯化钡(BaCl2,2μmol.L-1)、四乙胺(TEA,0.2 mol.L-1)、四氨基吡啶(4-AP,1 mmol.L-1)均不能抑制SPT的舒血管效应,ATP敏感钾通道(KATP)格列苯脲(Gli,10μmol.L-1)能抑制SPT对血管的舒张作用。在无钙的生理盐溶液中,SPT对CaCl2收缩血管有明显抑制作用。结论 SPT舒张血管的作用具有浓度依赖性,其作用机制可能与抑制Na+/H+交换、ATP敏感钾通道(KATP)以及钙离子内流有关。
Objective To investigate the effects of spironolactone (SPT) on the vascular smooth muscle tension in vitro and to explore the mechanism of action of SPT. Methods The in vitro vascular smooth muscle tension recording method was used to observe the effects of Sprague Dawley (SD) on isolated thoracic aorta rings and the effects of different tools on the use of SPT. Results SPT had a concentration-dependent relaxation effect on the pre-contracted vascular rings of KCl (30 mmol.L-1) and NE (1 μmol.L-1), with no difference in endothelium integrity and endothelium- Non-endothelium-dependent. Na + / H + exchange blocker amiloride (AM, 1μmol.L-1) inhibited the vasodilatation of SPT. Based on KCl precontraction, potassium channel blockers such as barium chloride (BaCl2, 2μmol.L-1), tetraethylammonium (TEA, 0.2mol·L-1), tetraaminopyridine .L-1) could not inhibit the vasodilator effect of SPT. ATP-sensitive potassium channel (KATP) glibenclamide (Gli, 10μmol.L-1) could inhibit the vasodilation of SPT. In calcium-free physiological saline solution, SPT significantly inhibited the contraction of CaCl2. Conclusions The effect of SPT on vasodilation is concentration-dependent. Its mechanism may be related to the inhibition of Na + / H + exchange, ATP-sensitive potassium channel (KATP) and calcium influx.