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目的 从HIV AIDS患者应用微量全血法分离中国HIV 1毒株 ,研究HIV 1的生物学特性与HIV AIDS疾病进展相关性。方法 建立微量全血法 ,从HIV AIDS全血标本中分离 17株HIV 1病毒分离株 ;检测这 17株病毒分离株嗜性和复制动力。结果 从 2 6例HIV AIDS病例中分离出HIV 1病毒 ,分离率为 6 5 .4 % (17 2 6 ) ,其中 17例HIV 1感染者的病毒分离率为 5 2 .9% (9 17) ,均为巨噬细胞嗜性 (M嗜性 ,NSI) ;9例AIDS患者的HIV 1病毒分离率为 88.9% (8 9) ,其中 7株为T细胞嗜性 (T嗜性 ,SI) ,1株为巨噬细胞嗜性。通过检测P2 4抗原确定 17株HIV 1病毒分离株的复制动力。在分离到的 17株HIV 1中 ,SI型病毒分离株与AIDS组显著相关 (P <0 .0 5 ) ;AIDS期的病毒分离株的复制动力明显高于HIV感染期 (P <0 .0 5 )。结论 微量全血法可用于病毒分离。 17株分离株的HIV 1复制动力与CD4 + T淋巴细胞计数呈线性负相关 ,与病毒载量呈正相关。
Objective To isolate Chinese HIV-1 strains from HIV-infected patients by micro-whole blood and investigate the relationship between the biological characteristics of HIV-1 and the progression of HIV / AIDS. Methods A total of 17 strains of HIV-1 isolates were isolated from HIV AIDS blood samples by micro-whole blood method. The motility and replication of these 17 isolates were tested. Results The HIV 1 virus was isolated from 26 HIV / AIDS cases and the isolation rate was 65.4% (17 2 6). The virus isolation rate of 17 HIV-1 infected persons was 52.9% (9 17) , All of which were macrophage tropism (M tropism, NSI). The AIDS virus isolation rate among 9 AIDS patients was 88.9% (8 9), of which 7 were T cell tropism (T tropism, SI) One strain is macrophage tropic. The replication motivity of 17 HIV 1 virus isolates was determined by detecting the P2 4 antigen. Among 17 isolated HIV-1 strains, the SI virus isolates were significantly associated with the AIDS group (P <0.05). The replication motifs of the AIDS virus isolates were significantly higher than those of the HIV-infected ones (P <0. 0) 5). Conclusion Micro-whole blood method can be used for virus isolation. There was a linear negative correlation between HIV-1 replication motility and CD4 + T lymphocyte counts in 17 isolates, which was positively correlated with viral load.