目的 :在培养的人内皮细胞上观察内皮素 (ET)、血管紧张素Ⅱ (AngⅡ )和同型半胱氨酸 (Hcy)对C -型利钠利尿肽 (CNP)生成和释放的影响 ,以探讨CNP生成和释放的机制。方法 :人内皮细胞培养 ;CNP放免测定。结果 :ET、AngⅡ均可刺激内皮细胞CNP的生成 ,10 -9、10 -8、10 -7mol/L的ET和AngⅡ分别使细胞CNP含量较对照组高 1% (P >0 .0 5 ) ,49% (P <0 .0 5 ) ,117% (P <0 .0 1)和 137% (P <0 .0 1) ,16 5 % (P <0 .0 1) ,2 0 1% (P <0 .0 1) ,大剂量ET、AngⅡ可刺激CNP的释放。Hcy对内皮细胞CNP的生成没有影响 ,但 10 -9、10 -8、10 -7mol/LHcy可使其释放增加 17% (P >0 .0 5 ) ,84% (P <0 .0 1)和 5 5 5 % (P <0 .0 1)。结论 :ET、Ang和Hcy可调节CNP的释放和 /或生成 Objective: To observe the effects of endothelin (ET), angiotensin Ⅱ (Ang Ⅱ) and homocysteine ​​(Hcy) on the formation and release of C - type natriuretic peptide (CNP) in cultured human endothelial cells To explore the mechanism of CNP generation and release. Methods: Human endothelial cell culture; CNP radioimmunoassay. Results: Both ET and AngⅡ stimulated CNP production in endothelial cells. ET-1 and AngⅡ at 10 -9, 10 -8 and 10 -7 mol / L increased CNP levels by 1% (P> 0.05) , 49% (P <0.05), 117% (P <0.01), 137% (P <0.01), 165% (P <0. 01), high-dose ET, Ang Ⅱ can stimulate the release of CNP. Hcy had no effect on CNP formation in endothelial cells, but 10 -9, 10 -8, 10 -7 mol / LHcy increased the release of CNP by 17% (P> 0.05) and 84% (P <0.01) And 55.5% (P <0.01). Conclusion: ET, Ang and Hcy regulate CNP release and / or production