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建立人血液中尼莫地平的气相色谱分析方法 ,扩大药物检测范围及检测手段 ,以适应法医学鉴定的需要。以NaNO2 为氧化剂 ,将尼莫地平完全氧化成其吡啶衍生物 (PA)后用GC ECD进行分析。以标准尼莫地平对方法的线性范围、精密度进行了测试 ;以人血浆标准添加尼莫地平对样品处理方法、回收率进行考察。所建方法的线性范围是 1 2 1～ 2 4 2ng·ml 1(γ =0 9993) ;最低检出限为 1 0mg·ml 1(S/N =3) ;日内与日间的变异系数分别为 (5 77± 2 31) %与 (5 5 3± 0 70 ) % (n =4) ;平均回收率为 91 0 %～ 99 9%。该方法可用于尼莫地平血药浓度监测及法医学鉴定。
To establish a method for the analysis of nimodipine in human blood by means of expanding the scope and means of drug testing to meet the needs of forensic identification. Nimodipine was completely oxidized to its pyridine derivative (PA) using NaNO2 as an oxidant and then analyzed by GC ECD. The standard nimodipine was used to test the linear range and precision of the method. The nimodipine was added to human plasma to evaluate the sample processing and recovery rate. The linear range of the proposed method was 1 2 1 ~ 2 4 2 ng · ml 1 (γ = 0 9993). The minimum detection limit was 10 mg · ml -1 (S / N = 3) (5 77 ± 2 31)% and (5 5 3 ± 0 70)% (n = 4) respectively. The average recoveries ranged from 91 0% to 99 9%. The method can be used for the monitoring of nimodipine blood concentration and forensic identification.