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在pH 7.0的HAc-NaAc缓冲液溶中,6-巯基嘌呤可以通过Au-S键自组装于金纳米粒子的表面。以Cu2+作为交联剂,Cu2+与6-巯基嘌呤间的配位作用导致金纳米粒子快速发生团聚,金纳米粒子溶液的颜色由红色变为蓝色,最大吸收波长从520nm红移至660nm。660nm和520nm处吸光度的比值(A660/A520)与6-巯基嘌呤的浓度在5.0×10-8—1.0×10-6moL/L范围内存在线性关系。该方法测定6-巯基嘌呤的检出限为1.4×10-8moL/L,相对标准偏差为2.9%(2.0×10-7mol/L 6-巯基嘌呤溶液,n=11)。该方法已用于人血清中的6-巯基嘌呤含量的测定。
In the HAc-NaAc buffer solution at pH 7.0, 6-mercaptopurine can be self-assembled on the surface of gold nanoparticles via Au-S bonds. The coordination between Cu2 + and 6-mercaptopurine led to rapid agglomeration of gold nanoparticles with Cu2 + as the crosslinking agent. The color of the gold nanoparticle solution changed from red to blue, and the maximum absorption wavelength was red-shifted from 520 nm to 660 nm. The ratio of absorbance at 660 nm and 520 nm (A660 / A520) had a linear relationship with the concentration of 6-mercaptopurine in the range of 5.0x10-8-1.0x10-6moL / L. The detection limit of 6-mercaptopurine in this method was 1.4 × 10-8moL / L with a relative standard deviation of 2.9% (2.0 × 10-7mol / L 6-mercaptopurine solution, n = 11). This method has been used for the determination of 6-mercaptopurine levels in human serum.