目的 :克隆人内皮抑素 (hES)全长cDNA ,并进行其在大肠杆菌表达的研究。方法 :用RT PCR从胎肝RNA中扩增hEScDNA片段 ,构建重组其克隆和融合蛋白表达载体 ,并进行克隆基因的DNA序列分析。结果 :克隆了hEScDNA ,其序列和国外报道一致 ;构建了重组hES融合蛋白表达菌株 ,表达目的蛋白达菌体总蛋白的5 0 %以上。结论 :通过hES基因克隆和表达的研究 ,获得了hES的基因克隆和高效表达菌株 Objective: To clone full length human endostatin (hES) cDNA and study its expression in E. coli. Methods: The hES cDNA fragment was amplified from fetal liver RNA by RT PCR. The recombinant cloned and fusion protein expression vector was constructed and the DNA sequence of the cloned gene was analyzed. Results: The hES cDNA was cloned and its sequence was consistent with those reported in foreign countries. The recombinant hES fusion protein was constructed and expressed at 50% of the total bacterial protein. Conclusion: The cloning and expression of hES gene obtained hES gene cloned and highly expressed strains