【摘 要】
：
获得Hsa-miR-1的方法主要由人工合成,需耗费很高的成本与时间.文章通过将Hsa-miR-1前体部分连接至pcDNA3.0,可实现Hsa-miR-1基因的高表达.构建含有Hsa-miR-1基因表达前体的表
【基金项目】
：
国家重点基础发展计划资助项目(973);
论文部分内容阅读
获得Hsa-miR-1的方法主要由人工合成,需耗费很高的成本与时间.文章通过将Hsa-miR-1前体部分连接至pcDNA3.0,可实现Hsa-miR-1基因的高表达.构建含有Hsa-miR-1基因表达前体的表达载体pcDNA-Hsa-miR-1和含有与Hsa-miR-1基因完全互补序列的检测载体pEGFP-C1-CM1.通过共转染pcDNA- Hsa-miR-1和pEGFP-C1-CM1检测Hsa-miR-1基因的表达,并采用Taqman探针法定量检测Hsa-miR-1基因表达水平,成功构建了可高效表达Hsa-miR-1基因的真核生物质粒pcDNA-Hsa-miR-1,并首次建立起Hsa-niR-1基因的检测系统pEGFP-C1-CM1,该检测系统的建立将为今后Hsa-miR-1在肿瘤细胞中的研究提供准确、快捷的检测方法.
其他文献
The reaction of triphenylphosphine with AgNO3 yielded an unusual nitrate anion bridging one-dimensional infinite chain [Ag(PPh3)NO3]∞ in which the silver(I) at
应用克隆的基因组序列作为同源臂,构建了小鼠β-酪蛋白基因定位敲人打靶载体.短臂长2.7kb,包括小鼠p-酪蛋白基因5端侧翼区、第1外显子、第1内含子、部分第2外显子.长臂包括小
Hydrophilic ZnS nanocrystals with narrow size distribution were synthesized via homogeneous precipitation using EDTA as stabilizer. The as-synthesized products
The utility of nickel/iron in the remediation of atrazine-contaminated water was investigated. The experimental results showed that nickel/iron had effective ca
This paper summarizes atmospheric aerosol concentrations of 5 stratospheric balloon soundings during the period from 1984 to 1994. Aerosol-rich layers in the tr
Program slicing can be effectively used to debug, test, analyze, understand and maintain objectoriented software. In this paper, a new slicing model is proposed
Some coupled 1D-2D hydrodynamic and water quality models depicting tidal water bodies with complex topography were presented. For the coupled models, finite ele
目的观察水通道蛋白1(AQP1)在小鼠口腔粘膜的表达与分布,为研究AQP1在口腔水代谢和感觉传递中的作用提供形态学依据。方法制备成年小鼠口腔各部粘膜石蜡切片,进行AQP1免疫组
目的:探讨A型核纤层蛋白前体( prelamin A)在细胞内堆积造成细胞早老的机理,筛选了prelamin A相互作用蛋白并研究其在早老细胞中的表达情况.方法:以prelamin A的C末端区域为
The adsorption of two phenols, namely, phenol and salicylic acid(SA) onto a water-compatible hypercrosslinked polymeric resin(NJ-8) were studied in terms of pse