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目的:探讨黑木耳多糖对内毒素(LPS)诱导大鼠急性肾损害的保护效应及可能机制。方法:健康雄性SD大鼠,分为正常对照组、LPS组和黑木耳多糖组(LPS+黑木耳多糖)。根据分组,分别于复制模型前7 d给予生理盐水或1%黑木耳多糖(15 m L/kg)预防性灌胃。第8 d腹腔注射生理盐水或LPS(8 mg/kg),造模12 h后,通过腹主动脉采血检测血清尿素氮(BUN)、肌酐(Cr)水平,取肾脏制备匀浆检测肾组织中丙二醛(MDA)、超氧化物歧化酶(SOD)、总抗氧化能力(T-AOC)水平。结果:用黑木耳多糖干预后,大鼠血清中BUN、Cr水平均显著低于LPS组(P<0.05);LPS组MDA含量较正常对照组增高,SOD、T-AOC水平降低,差异有统计学意义(P<0.05);黑木耳多糖组MDA含量较LPS组降低,SOD、T-AOC水平升高,差异有统计学意义(P<0.05)。结论:黑木耳多糖能显著提高内毒素血症大鼠的抗氧化能力,对肾脏组织有保护作用。
Objective: To investigate the protective effect of black fungus polysaccharide on acute renal injury induced by endotoxin (LPS) in rats and its possible mechanism. Methods: Healthy male Sprague-Dawley rats were divided into normal control group, LPS group and black fungus polysaccharide group (LPS + black fungus polysaccharide). Groups were given prophylactic gavage with saline or 1% Auricularia polysaccharides (15 m L / kg) 7 d before the replicate model. The rats were injected intraperitoneally with normal saline or LPS (8 mg / kg) on the 8th day. After 12 h of modeling, blood urea nitrogen (BUN) and creatinine (Cr) were measured by blood sampling in the abdominal aorta. Malondialdehyde (MDA), superoxide dismutase (SOD) and total antioxidant capacity (T-AOC). Results: The levels of BUN and Cr in serum of rats were significantly lower than those of LPS group (P <0.05). The content of MDA in LPS group was higher than that in normal control group, and the levels of SOD and T-AOC were decreased (P <0.05). The content of MDA in the black fungus polysaccharide group was lower than that in the LPS group and the level of SOD and T-AOC was increased. The difference was statistically significant (P <0.05). Conclusion: Auricularia polysaccharides can significantly enhance the antioxidant capacity of endotoxemia rats and protect the kidney tissue.