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目的检测呼吸道合胞病毒感染毛细支气管炎患儿血清结合膜蛋白细胞分化抗原14(s CD14)、Toll样受体4(TLR4)及核苷酸结合寡聚域样受体2(NLR2)的表达,探讨模式识别受体在毛细支气管炎发病机制中的作用。方法采集2013年-2014年间住院治疗的45例毛细支气管炎患儿静脉血,酶联免疫吸附试验(ELISA)法检测血清s CD14、TLR4和NLR2的蛋白浓度,并与35例健康儿童作对照。结果毛细支气管炎组血清s CD14〔(2.46±1.10)ng/ml〕、TLR4〔(34.56±13.99)ng/ml〕和NLR2〔(72.31±30.04)ng/ml〕蛋白的浓度均显著高于对照组〔(1.45±0.61)ng/ml,(16.23±7.43)ng/ml,(31.54±10.51)ng/ml〕两组比较,差异有统计学意义(t=5.18、7.53、8.46,P均<0.01)。TLR4和NLR2的表达呈显著直线正相关(r=0.58,P<0.01)。结论模式识别受体s CD14、TLR4和NLR2在毛细支气管炎患儿血清中表达升高,他们在清除呼吸道合胞病毒过程中具有协同作用,可能被作为评价病情变化的炎症指标之一。
Objective To detect the expression of sCD14, TLR4 and NLR2 in children with bronchiolitis with respiratory syncytial virus infection , To explore the role of pattern recognition receptors in the pathogenesis of bronchiolitis. Methods Venous blood was collected from 45 children with bronchiolitis treated in our hospital from 2013 to 2014. Serum levels of CD14, TLR4 and NLR2 were detected by enzyme-linked immunosorbent assay (ELISA) and compared with 35 healthy children. Results The serum levels of CD14 (2.46 ± 1.10) ng / ml, TLR4 〔(34.56 ± 13.99) ng / ml〕 and NLR2 〔(72.31 ± 30.04) ng / ml〕 in patients with bronchiolitis were significantly higher than those in the control (1.45 ± 0.61) ng / ml, (16.23 ± 7.43) ng / ml, (31.54 ± 10.51) ng / ml], the difference was statistically significant (t = 5.18,7.53,8.46, P < 0.01). TLR4 and NLR2 expression showed a significant positive linear correlation (r = 0.58, P <0.01). Conclusions The expression of pattern recognition receptors CD14, TLR4 and NLR2 in the serum of children with bronchiolitis is increased. They may play synergistic roles in the clearance of respiratory syncytial virus and may be used as an index of inflammation in evaluating the disease.